Flowing through Gastrointestinal Barriers with Model Nanoparticles: From Complex Fluids to Model Human Intestinal Epithelium Permeation

Author(s)

Ribeiro, Iris Renata Sousa ; da Silva, Raquel Frenedoso ; Rabelo, Renata Santos ; Marin, Talita Miguel ; Bettini, Jefferson ; Cardoso, Mateus Borba

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 30, p. 11-pg., 2023-07-19.

Abstract

Most nanomaterial-based medicines are intravenously appliedsinceoral administration comprises challenging-related biological obstacles,such as interactions with distinct digestive fluids and their transportthrough the intestinal barrier. Moreover, there is a lack of nanoparticle-basedstudies that faithfully consider the above-cited obstacles and boostoral-administered nanomedicines' rational design. In this study,the physicochemical stability of fluorescent model silica nanoparticles(f-SiO(2)NPs) passing through all simulated gastrointestinalfluids (salivary, gastric, and intestinal) and their absorption andtransport across a model human intestinal epithelium barrier are investigated.An aggregation/disaggregation f-SiO(2)NPs process is identified,although these particles remain chemically and physically stable afterexposure to digestive fluids. Further, fine imaging of f-SiO(2)NPs through the absorption and transport across the human intestinalepithelium indicates that nanoparticle transport is time-dependent.The above-presented protocol shows tremendous potential for decipheringfundamental gastrointestinal nanoparticles' evolution and cancontribute to rational oral administration-based nanomedicine design. (AU)

• FAPESP's process: 21/12071-6 - Tailoring colloids through supramolecular interactions: from fundamentals to applications
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/21318-0 - Targeting of functionalized nanoparticles in microfluidics systems containing multiple organoids
• Grantee: Iris Renata Sousa Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate