Comparison of the acyclovir release carried in chitosan-based microstructured systems

Author(s)

da Silva, Bruno Batista ; Menezes, Jorge Eduardo ; da Costa, Mardoqueu Martins ; dos Santos, Laurita ; Sanfelice, Rafaela Cristina ; Pavinatto, Adriana

• Document type: Journal article
• Source: POLYMER BULLETIN; v. N/A, p. 15-pg., 2023-08-11.

Abstract

Herpes simplex virus infections are caused by two types (1 and 2) of the virus, being endemic worldwide and a frequent public health concern. For treatment, the drug of first choice is acyclovir (ACV), which acts by inhibiting viral DNA polymerase. However, its topical use in the form of ointments requires frequent application to achieve the desired efficacy, as it has low permeation. In this context, the use of biomaterials such chitosan (CS) as a carrier in the controlled release of drugs is an interesting alternative in health area. In the present work, CS-based membranes, microcapsules, and membrane/microcapsules were produced and characterized for encapsulation and release of the ACV. The membranes were produced using the casting technique, and the microcapsules were obtained through ionotropic gelation. The microstructures were characterized by infrared spectroscopy (FTIRATR), X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, mass loss and gain through water absorption tests, mechanical analysis, and release tests. Membranes with 23-33 +/- 3 mu m of thickness and microcapsules with similar to 1 mm of diameter were produced. FTIR spectra shows the typical vibrational regions related to the formed materials chemical groups. The found modulus of elasticity was 2.2 x 10(3), 1.5 x 10(3) and 1.1 x 10(3) MPa for CS, CS/glycerol 30% and CS/glycerol 30%/ACV membranes, respectively, suggesting higher rigidity for pure CS and least rigidity for ACV-containing membranes. TGA results show that all membranes are thermally stable up to 200 degrees C. The microcapsules and membrane/microcapsules systems showed better stability in drug encapsulation. Regarding to release assay, all microstructures demonstrate total ACV release, being calculated 101.5, 93.8, and 107.2% of concentration released for membrane, microcapsules, and membrane/capsules systems, respectively. The membrane/microcapsules modulated the release in 1 h and 20 min, with peak concentration at 2 h, while membrane and capsule systems had the entire drug released after 15/20 min. (AU)

• FAPESP's process: 17/19470-8 - Development of portable biosensor for non-invasive detection of uric acid as indicative of hyperuricemia
• Grantee: Adriana Pavinatto da Costa
• Support Opportunities: Regular Research Grants