| Full text | |
| Author(s): |
Acrani, Gustavo Olszanski
;
Tilston-Lunel, Natasha L.
;
Spiegel, Martin
;
Weidmann, Manfred
;
Dilcher, Meik
;
Andrade da Silva, Daisy Elaine
;
Nunes, Marcia R. T.
;
Elliott, Richard M.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | JOURNAL OF GENERAL VIROLOGY; v. 96, p. 11-pg., 2015-03-01. |
| Abstract | |
Oropouche virus (OROV) is a medically important orthobunyavirus, which causes frequent outbreaks of a febrile illness in the northern parts of Brazil. However, despite being the cause of an estimated half a million human infections since its first isolation in Trinidad in 1955, details of the molecular biology of this tripartite, negative-sense RNA virus remain limited. We have determined the complete nucleotide sequence of the Brazilian prototype strain of OROV, BeAn 19991, and found a number of differences compared with sequences in the database. Most notable were that the S segment contained an additional 204 nt at the 3' end and that there was a critical nucleotide mismatch at position 9 within the base-paired terminal panhandle structure of each genome segment. In addition, we obtained the complete sequence of the Trinidadian prototype strain TRVL-9760 that showed similar characteristics to the BeAn 19991 strain. By using a 17 RNA polymerase-driven minigenome system, we demonstrated that cDNA clones of the BeAn 19991 L and S segments expressed functional proteins, and also that the newly determined terminal untranslated sequences acted as functional promoters in the minigenome assay. By co-transfecting a cDNA to the viral glycoproteins, virus-like particles were generated that packaged a minigenome and were capable of infecting naive cells. (AU) | |
| FAPESP's process: | 13/02798-0 - Reverse genetics of Oropouche virus: generation of mutants with truncated and deleted non structural NSs protein |
| Grantee: | Gustavo Olszanski Acrani |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |