Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism

Author(s):Show less -

Araujo, Bruno C. ; Barbosa, David Aciole ; Honji, Renato M. ; Branco, Giovana S. ; Menegidio, Fabiano B. ; Marques, Victor H. ; Moreira, Renata G. ; Kitahara, Marcelo V. ; Rombenso, Artur N. ; de Mello, Paulo H. ; Hilsdorf, Alexandre W. S.

• Document type: Journal article
• Source: MARINE BIOTECHNOLOGY; v. 25, n. 3, p. 14-pg., 2023-05-10.

Abstract

We used transcriptome sequencing to investigate the hepatic postprandial responses of Rachycentron canadum (cobia), an important commercial fish species. In total, 150 cobia juveniles (50 per tank, triplicate) were fed ad libitum with a commercial diet for 7 days, fasted for 24 h, and fed for 10 min. The liver was sampled 10 min prior to feeding and 30 min, 1, 2, 4, 8, 12, and 24 h after the feeding event. Each sample was evaluated in terms of liver fatty acid profile and gene expression. Differential gene expressions were evaluated, focusing on fatty acid synthesis and oxidation pathways. In general, the liver fatty acid profile reflected diet composition. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) levels increased at 8 to 12 h but decreased at 24 h after the feeding event. A high number of differentially expressed genes (DEGs) were observed comparing fish that fasted for 8 h with those fasted for 30 min and 24 h, while a reduced number of DEGs was observed comparing individuals who fasted for 30 min compared with those who fasted for 24 h. Similarly, the main differences in the expression of genes related to the fatty acid biosynthesis and oxidation pathways were noticed in individuals who fasted for 8 h compared with those who fasted for 30 min and 24 h. The results suggested that the adequate time to sample the individuals ranged between 8 and 12 h after the meal since, apparently, after 24 h, differential gene expression was not necessarily influenced by food intake. (AU)

• FAPESP's process: 19/26018-0 - Lipid metabolism of bijupirá, Rachycentron canadum juveniles during the postprandial period and using alternative fatty acid sources
• Grantee: Renata Guimarães Moreira Whitton
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/12435-0 - Influence of the temperature and diets with different DHA/ARA ratio in the transcriptome response, lipid metabolism and performance of cobia juveniles (Rachycentron canadum)
• Grantee: Bruno Cavalheiro Araújo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral