Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance

Author(s)

Kordalewska, Milena ; Cancino-Prado, Geselle ; de Almeida Jr, Joao Nobrega ; Brandao, Igor Brasil ; Peral, Renata Tigulini de Souza ; Colombo, Arnaldo. L. L. ; Perlin, David. S. S.

• Document type: Journal article
• Source: Antimicrobial Agents and Chemotherapy; v. 67, n. 6, p. 4-pg., 2023-05-24.

Abstract

We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32x; caspofungin, >64x; micafungin, >64x). We determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. (AU)

• FAPESP's process: 17/02203-7 - Multidisciplinary and international network to characterize microbiological aspects and natural history of invasive fungal infections due Candida spp
• Grantee: Arnaldo Lopes Colombo
• Support Opportunities: Research Projects - Thematic Grants