Retinal organoids from human-induced pluripotent stem cells: From studying retinal dystrophies to early diagnosis of Alzheimer?s and Parkinson?s disease

Author(s)

Movio, Marilia Ines ; de Lima-Vasconcellos, Theo Henrique ; dos Santos, Gabrieli Bovi ; Echeverry, Marcela Bermudez ; Colombo, Elisabetta ; Mattos, Leonardo S. ; Resende, Rodrigo Ribeiro ; Kihara, Alexandre Hiroaki

• Document type: Journal article
• Source: SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY; v. 144, p. 10-pg., 2023-03-17.

Abstract

Human-induced pluripotent stem cells (hiPSCs) have provided new methods to study neurodegenerative dis-eases. In addition to their wide application in neuronal disorders, hiPSCs technology can also encompass specific conditions, such as inherited retinal dystrophies. The possibility of evaluating alterations related to retinal disorders in 3D organoids increases the truthfulness of in vitro models. Moreover, both Alzheimer's (AD) and Parkinson's disease (PD) have been described as causing early retinal alterations, generating beta-amyloid protein accumulation, or affecting dopaminergic amacrine cells. This review addresses recent advances and future perspectives obtained from in vitro modeling of retinal diseases, focusing on retinitis pigmentosa (RP). Additionally, we depicted the possibility of evaluating changes related to AD and PD in retinal organoids ob-tained from potential patients long before the onset of the disease, constituting a valuable tool in early diagnosis. With this, we pointed out prospects in the study of retinal dystrophies and early diagnosis of AD and PD. (AU)

• FAPESP's process: 21/14227-3 - ESTABLISHMENT OF A 3D IN VITRO MODEL OF RETINITIS PIGMENTOSA
• Grantee: Gabrieli Bovi dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 21/03711-1 - Analysis of the consequences of Argonauta-2 protein knockdown on synaptogenesis in vitro
• Grantee: Gabrieli Bovi dos Santos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/17892-8 - Degeneration and development of the nervous system: the role of epigenetic processes
• Grantee: Alexandre Hiroaki Kihara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/02035-0 - Descriptive and functional analysis of the IP3R1 receptor in the pathology of retinitis pigmentosa using C3H/HeJ mice model
• Grantee: Théo Henrique de Lima Vasconcellos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/11667-0 - Cellular and electrophysiological changes in the hippocampus caused by neurodegenerative processes related to neonatal anoxia and temporal lobe epilepsy
• Grantee: Alexandre Hiroaki Kihara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/11969-9 - The role of IP3R1 in neuroinflammation triggered by retinitis pigmentosa
• Grantee: Théo Henrique de Lima Vasconcellos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/26388-6 - Functional expression of AGO2: study of the 'core of risc complex' role in retinal development
• Grantee: Marília Inês Móvio
• Support Opportunities: Scholarships in Brazil - Master