Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70): Application in diagnosis/prognosis of coronavirus disease 2019 (COVID-19)

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Negahdary, Masoud ; Hirata, Mario Hiroyuki ; Sakata, Solange Kazumi ; Ciconelli, Rozana Mesquita ; Bastos, Gisele Medeiros ; Borges, Jessica Bassani ; Thurow, Helena Strelow ; Junior, Alceu Totti Silveira ; Sampaio, Marcelo Ferraz ; Guimaraes, Larissa Berretta ; Maeda, Bruno Sussumu ; Angnes, Lucio

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 1242, p. 10-pg., 2023-02-15.

Abstract

In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic per-formance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL(-1) to 75 ng mL(-1), with a limit of detection (LOD) of similar to 2 pg mL(-1). The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method. (AU)

• FAPESP's process: 19/27021-4 - Design and fabrication of electrochemical biosensors for early diagnosis of myocardial infarction and Hepatocellular Carcinoma using microRNAs (miRNAs) primers as biorecognition element and arrays of gold nanostructures
• Grantee: Masoud Negahdary
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants