Growing kinetics and antimicrobial activity of Streptomyces tubercidicus crude extracts

Author(s)

Priscila Ratti, R. ; Toledo Piza, A. C. M. ; Granato, A. C. ; Hokka, C. O. ; Dubreuil, J. D. ; Paiva de Sousa, C. ; MendezVilas, A

• Document type: Journal article
• Source: MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS; v. N/A, p. 4-pg., 2011-01-01.

Abstract

Streptomyces spp. is studied in function of its antibiotic production with industrial and pharmaceutical applications. This work aimed to evaluate the growing kinetics of the endophytic S. tubercidicus isolated from Solanum lycocarpum St. Hill, a typical Brazilian tropical savannah tree, and compares its bioactivity against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922. S. tubercidicus was cultivated in ISP2 broth and its growing kinetics was evaluated during 21 days. The major quantification measured was 7.88 log CFU.m1(-1) seen at the second day of incubation. The bioactive substances production was seen at the end of the exponential phase and the beginning of the stationary phase of growth (day two). The fresh crude extract showed a maximum antimicrobial potential of 200 AU.mL(-1) against Escherichia coli and Staphylococcus aureus. The crude extract was active during 21 days, without modification of its antimicrobial potential and revealed three compounds using thin layer chromatography under UV illumination. (AU)

• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants