Flow Cytometry as a Tool for Analyzing Changes in Plasmodium falciparum Cell Cycle Following Treatment with Indol Compounds

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Author(s):	Schuck, Desiree Cigaran ; Ribeiro, Ramira Yuri ; Nery, Arthur A. ; Ulrich, Henning ; Garcia, Celia R. S. Total Authors: 5
Document type:	Journal article
Source:	Cytometry Part A; v. 79A, n. 11, p. 6-pg., 2011-11-01.
Abstract
Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. (C) 2011 International Society for Advancement of Cytometry (AU)

FAPESP's process:	07/52924-0 - Functional genomics in Plasmodium
Grantee:	Célia Regina da Silva Garcia
Support Opportunities:	Research Projects - Thematic Grants