Human leukocyte antigen-G 3 ' untranslated region polymorphisms are associated with better kidney allograft acceptance

Author(s)

Ciliao Alves, Daiani Cristina ; de Oliveira Crispim, Janaina Cristiana ; Castelli, Erick C. ; Mendes-Junior, Celso Teixeira ; Saloun Deghaide, Neifi Hassan ; Barros Silva, Gyl Eanes ; Costa, Roberto Silva ; Saber, Luciana Tanajura ; Moreau, Philippe ; Donadi, Eduardo Antonio

• Document type: Journal article
• Source: HUMAN IMMUNOLOGY; v. 73, n. 1, p. 8-pg., 2012-01-01.

Abstract

Human leukocyte antigen-G (FILA-G) plays a well-recognized role in the modulation of the immune response, and HLA-G expression has been associated with increased graft survival and decreased rejection episodes. To investigate the role of the HLA-G 3' untranslated region (3'UTR) in renal transplantation, we evaluated several polymorphic sites (14-bp Del/Ins +3003T/C, +3010C/G, +3027C/A, +3035C/T, +3142G/C, and +3187A/G) in patients exhibiting or not exhibiting rejection episodes. A total of 104 patients (15 with acute and 48 with chronic rejection, and 41 with no rejection) and 142 healthy individuals were studied. HLA-G 3'UTR was typed by direct sequencing. The +3035C-C genotype was more frequent in patients exhibiting chronic rejection compared with healthy controls, and the +3035C-T genotype was less frequent in chronic rejection compared with patients without rejection (acute plus chronic) or compared with healthy controls. The +3187G-A genotype, in which the A allele is associated with increased mRNA degradation, showed increased frequency in the rejection group (acute plus chronic) when compared with healthy controls. The 14 base pair Deletion/Insertion genotype was marginally increased in patients with acute rejection. This is the first study to show associations among numerous polymorphic sites in the HLA-G 3'UTR in kidney allotransplantation, which may contribute to the understanding of HLA-G post-transcriptional mechanisms. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 10/14946-5 - mRNAs, miRNAs and Regulatory Molecules Expression profile in the Immune Response in Renal Allograft
• Grantee: Daiani Cristina Cilião Alves
• Support Opportunities: Scholarships in Brazil - Doctorate