Crystallization and preliminary X-ray analysis of stationary phase survival protein E (SurE) from Xylella fastidiosa in two crystal forms

Author(s)

dos Reis, Marcelo Augusto ; Saraiva, Antonio Marcos ; dos Santos, Marcelo Leite ; de Souza, Anete Pereira ; Aparicio, Ricardo

• Document type: Journal article
• Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 68, p. 4-pg., 2012-04-01.

Abstract

The bacterium Xylella fastidiosa is a phytopathogenic organism that causes citrus variegated chlorosis, a disease which attacks economically important crops, mainly oranges. In this communication, the crystallization and preliminary X-ray crystallographic analysis of XfSurE, a survival protein E from X. fastidiosa, are reported. Data were collected for two crystal forms, I and II, to 1.93 and 2.9 angstrom resolution, respectively. Crystal form I belonged to space group C2, with unit-cell parameters a = 172.36, b = 84.18, c = 87.24 angstrom, alpha = gamma = 90, beta = 96.59 degrees, whereas crystal form II belonged to space group C2, with unit-cell parameters a = 88.05, b = 81.26, c = 72.84 angstrom, alpha = gamma = 90, beta = 94.76 degrees. (AU)