Solanum pennellii LA716 as a Source of Genes Improving In Vitro Organogenesis in Cultivated Tomato

Author(s)

Arikita, F. N. ; Azevedo, M. S. ; Peres, L. E. P. ; Scotton, D. C. ; Figueira, A. ; Geelen, D

• Document type: Journal article
• Source: VII INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING; v. 961, p. 7-pg., 2012-01-01.

Abstract

In the present work we are reporting the high in vitro regeneration capacity of the tomato related wild species S. pennellii LA716, which enabled us to used a collection of 50 introgression lines (ILs), each containing small chromosomal segments of LA716 introgressed and mapped into the cultivar 'M82'. We found a high shoot regeneration capacity for IL3-2, IL6-1, IL7-1 (7-2, 7-3), IL 8-3 (8-2), IL-9-1 (9-2) and IL10-2 (10-3), when 12-days-old cotyledon explants were cultivated in MS medium containing 5.0 mu M BAP. This means that S. pennellii probably presents superior alleles for in vitro regeneration in such chromosomal segments. Since ILs 3-2, 7-1, 8-3, and 10-2 also presented enhanced root formation in MS medium containing 0.4 mu M NAA, they may represent novel alleles controlling the competence to assume different cell fates, rather than the induction of a specific organ. The alleles discovered here will provide for the characterization and isolation of important genes for plant development studies and biotechnological applications. (AU)

• FAPESP's process: 07/07175-0 - Genetic analysis of Theobroma cacao defense response against Moniliophthora perniciosa, the causal agent of witches broom
• Grantee: Antonio Vargas de Oliveira Figueira
• Support Opportunities: Research Projects - Thematic Grants