Biodegradation of caries-affected dentin bonding interface of fluoride and MDPB-containing adhesive system

Author(s)

Carvalho, Fabiola G. ; Carlo, Hugo L. ; Sacramento, Patricia A. ; de Barros, Silvio ; Santos, Rogerio L. ; Puppin-Rontani, Regina M.

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES; v. 47, p. 7-pg., 2013-12-01.

Abstract

The aim of this study was to evaluate the effect of a fluoride/MDPB (12-methacryloyloxydodecylpyridinium bromide)-containing adhesive system on the durability of a bond to permanent artificially induced caries-affected dentin (CAD) exposed to Streptococcus mutans culture and water storage. Twelve third molars were selected. Flat dentin surfaces were submitted to artificial caries development in S. mutans and Broth heart infusion (BHI). Caries-infected dentin was removed with burs according to clinical criteria and CAD cavities were restored with Adper Scotchbond Multi-Purpose (SBM) and Clearfil Protect Bond (CPB). Nontrimmed resin-dentin bonded interfaces (1 mm(2)) were stored in S. mutans+BHI for 3 days, in deionized water for 3 months, and afterwards subjected to microtensile bond strength test (mu TBS). The control group was not submitted to storage and immediate mu TBS testing was performed. Fractographic analysis was performed after mu TBS testing. Four molars were restored as described, and morphological evaluation hybrid layer (HL) was performed by scanning electron microscopy (SEM). Two-way ANOVA with split-plot design and Tukey's tests were performed. No difference was found between mu TBS values of SBM and CPB irrespective of groups. Significant decrease was observed in mu TBS values after S. mutans culture and water storage, but without difference between them. CPB had more homogenous hybrid layer than SBM. Fluoride/MDPB-containing adhesive system did not prevent degradation of CAD bond strength in both degradation methods. (C) 2013 Elsevier Ltd. All rights reserved. (AU)