| Full text | |
| Author(s): |
Merino, Victor R. C.
;
Nakano, Viviane
;
Finegold, Sydney M.
;
Avila-Campos, Mario J.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | SCIENTIFICA; v. 2014, p. 4-pg., 2014-01-01. |
| Abstract | |
The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples fromchildren with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB- and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains. (AU) | |
| FAPESP's process: | 07/03655-7 - Quantitative evaluation of Bacteroides fragilis enterotoxigenic strains and respective BFT gene subtypes in immunocompetent children with and without acute diarrhea |
| Grantee: | Victor Rafael Castillo Merino |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 09/03792-0 - Expression of Bacteroides fragilis bmeBAC efflux pump genes in Escherichia coli and characterization of the substrate profiles of the BmeABC pumps |
| Grantee: | Viviane Nakano |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |