Stability and in vitro antimicrobial efficacy of a nanopropolis formulation intended for intramammary treatment of bovine mastitis

Author(s)

Troncarelli, Marcella Z. ; Langoni, Helio ; Brandao, Humberto de Mello ; Dimkic, Ivica ; Stankovic, Slavisa ; Ribeiro, Andrea Rentz

• Document type: Journal article
• Source: BRAZILIAN JOURNAL OF HYGIENE AND ANIMAL SANITY; v. 8, n. 5, p. 8-pg., 2014-09-01.

Abstract

Organic dairy farms are supposed to use only phytotherapeutical and homeopatical actives for mastitis control in cows. However, there are a low number of actives available for this application. Considering the bactericide action of the propolis and the fact that this substance is allowed for use in organic dairies, Brazilian researchers developed the nanostructured propolis, a promising product for intramammary treatment against mastitis. The aim of the present study was to evaluate the stability of this nanopropolis formulation [30.2 mg/mL] stored at -20 degrees C, 8 degrees C, 25 degrees C and 37 degrees C during 90 days, and also the minimum inhibitory concentration (MIC) against ATCCs and bacterial field strains isolated from clinical and subclinical bovine mastitis cases in dairy herds from Sao Paulo and Minas Gerais states, Brazil. MIC values were obtained by microdilution test, using Mueller Hinton broth and resazurin dye. Mean of MIC values of nanopropolis against all tested strains ranged from 0.02 to 0.37 mg/mL. Product in vitro efficacy remained stable at different temperatures during all evaluation period. The obtained stability and in vitro efficacy results indicate that the evaluated nanopropolis formulation may represents a new and important therapeutic alternative for mastitis control, especially in organic dairy herds. (AU)

• FAPESP's process: 12/03212-6 - Nanotechnology in farming: use of a nanopropolis formulation as a therapeutical alternative for bovine mastitis control in organic dairy farms
• Grantee: Marcella Zampoli Troncarelli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral