Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis

Author(s):Show less -

Watanabe Ishikawa, Larissa Lumi ; Colavite, Priscila Maria ; de Campos Fraga-Silva, Thais Fernanda ; Nishiyama Mimura, Luiza Ayumi ; Donega Franca, Thais Graziela ; Goncalves Zorzella-Pezavento, Sofia Fernanda ; Chiuso-Minicucci, Fernanda ; Doddi Marcolino, Larissa ; Marques, Camila ; Valerio Ikoma, Maura Rosane ; Sartori, Alexandrina

• Document type: Journal article
• Source: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2016, p. 11-pg., 2016-01-01.

Abstract

This study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 mu g or 100 mu g) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-gamma and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cellsmaturation this procedure did not alter the frequency of regulatory Foxp3(+) T cells. Lower TNF-alpha and IL-6 levels and higher expression of ROR-gamma and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis. (AU)

• FAPESP's process: 11/17325-4 - Immunomodulatory strategies in experimental arthritis based on induction of specific tolerance by vitamin D3 and rapamycin
• Grantee: Alexandrina Sartori
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/20470-3 - Specific tolerogenic strategies for prophylaxis and therapy in experimental autoimmune arthritis.
• Grantee: Larissa Lumi Watanabe Ishikawa
• Support Opportunities: Scholarships in Brazil - Doctorate