| Full text | |
| Author(s): Show less - |
Orcia, Debora
;
Zeraik, Ana Eliza
;
Lopes, Jose L. S.
;
Macedo, Joci N. A.
;
dos Santos, Clarissa Romano
;
Oliveira, Katia C.
;
Anderson, Leticia
;
Wallace, B. A.
;
Verjovski-Almeida, Sergio
;
Araujo, Ana P. U.
;
DeMarco, Ricardo
Total Authors: 11
|
| Document type: | Journal article |
| Source: | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1861, n. 1, p. 8-pg., 2017-01-01. |
| Abstract | |
Background: The Micro-Exon Gene-14 (MEG-14) displays a remarkable structure that allows the generation of antigenic variation in Schistosomes. Previous studies showed that the soluble portion of the MEG-14 protein displays features of an intrinsically disordered protein and is expressed exclusively in the parasite esophageal gland. These features indicated a potential for interaction with host proteins present in the plasma and cells from ingested blood. Methods: A yeast two-hybrid experiment using as bait the soluble domain of Schistosoma mansoni MEG-14 (sMEG-14) against a human leukocyte cDNA library was performed. Pull-down and surface plasmon resonance (SPR) experiments were used to validate the interaction between sMEG-14 and human S100A9. Synchrotron radiation circular dichroism (SRCD) were used to detect structural changes upon interaction between sMEG-14 and human S100A9. Feeding of live parasites with S100A9 attached to a fluorophore allowed the tracking of the fate of this protein in the parasite digestive system. Results: S100A9 interacted with sMEG-14 consistently in yeast two-hybrid assay, pull-down and SPR experiments. SRCD suggested that MEG-14 acquired a more regular structure as a result of the interaction with S100A9. Accumulation of recombinant S100A9 in the parasite's esophageal gland, when ingested by live worms suggests that such interaction may occur in vivo. Conclusion: S100A9, a protein previously described to be involved in modulation of inflammatory response, was found to interact with sMEG-14. General significance: Our results allow proposing a mechanism involving MEG-14 for the parasite to block inflammatory signaling, which would occur upon release of S100A9 when ingested blood cells are lysed. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/07288-7 - Study of structure, immunoreactivity and protein partners of proteins encoded by micro-exon genes of Schistosoma mansoni |
| Grantee: | Débora Orcia |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/20715-4 - Studies of the correlation between septin dynamics and Ca2+ homeostasis in muscular cells from Schistosoma mansoni |
| Grantee: | Ana Eliza Zeraik |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/09361-9 - Study of micro-exon genes (MEGs) of the human parasite Schistosoma mansoni and of the interaction of their protein products with human cells |
| Grantee: | Ricardo de Marco |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/09186-7 - Study of micro-exon genes (MEGs) from the human parasite Schistosoma mansoni and its protein products |
| Grantee: | Ricardo de Marco |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/20290-5 - Structural studies of human septins complex |
| Grantee: | Joci Neuby Alves Macedo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |