| Full text | |
| Author(s): |
Blandenier, Quentin
;
Lara, Enrique
;
Mitchell, Edward A. D.
;
Alcantara, Daniel M. C.
;
Siemensma, Ferry J.
;
Todorov, Milcho
;
Lahr, Daniel J. G.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | EUROPEAN JOURNAL OF PROTISTOLOGY; v. 58, p. 12-pg., 2017-04-01. |
| Abstract | |
Molecular phylogeny is an indispensable tool for assessing evolutionary relationships among protists. The most commonly used marker is the small subunit ribosomal RNA gene, a conserved gene present in many copies in the nuclear genomes. However, this marker is not variable enough at a fine-level taxonomic scale, and intra-genomic polymorphism has already been reported. Finding a marker that could be useful at both deep and fine taxonomic resolution levels seemed like a utopic dream. We designed Amoebozoa-specific primers to amplify a region including partial sequences of two subunits of the mitochondrial nicotinamide adenine dinucleotide dehydrogenase gene (NAD9/NAD7). We applied them to arcellinids belonging to distantly related genera (Arcella, Difflugia, Netzelia and Hyalosphenia) and to Arcellinid-rich environmental samples to obtain additional Amoebozoa sequences. Tree topology was congruent with previous phylogenies, all nodes being highly supported, suggesting that this marker is well-suited for deep phylogenies in Arcellinida and perhaps Amoebozoa. Furthermore, it enabled discrimination of close-related taxa. This short genetic marker (ca. 250 bp) can therefore be used at different taxonomic levels, due to a fast-varying intergenic region presenting either a small intergenic sequence or an overlap, depending on the species. (C) 2016 Elsevier GmbH. All rights reserved. (AU) | |
| FAPESP's process: | 13/04585-3 - Deciphering the major trends of molecular and morphological evolution in the Amoebozoa |
| Grantee: | Daniel José Galafasse Lahr |
| Support Opportunities: | Research Grants - Young Investigators Grants |