| Full text | |
| Author(s): |
Garcia, J. A.
;
Ferreira, H. L.
;
Vieira, F. V.
;
Gameiro, R.
;
Andrade, A. L.
;
Eugenio, F. R.
;
Flores, E. F.
;
Cardoso, T. C.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | VETERINARY AND COMPARATIVE ONCOLOGY; v. 15, n. 2, p. 9-pg., 2017-06-01. |
| Abstract | |
Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication-induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres (p<0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy. (AU) | |