| Full text | |
| Author(s): Show less - |
Scarel-Caminaga, Raquel M.
;
Cera, Flavia F.
;
Pigossi, Suzane C.
;
Finoti, Livia S.
;
Kim, Yeon J.
;
Viana, Aline C.
;
Secolin, Rodrigo
;
Montenegro, Marcelo F.
;
Tanus-Santos, Jose E.
;
Orrico, Silvana R. P.
;
Cirelli, Joni A.
Total Authors: 11
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 18, n. 6, p. 11-pg., 2017-06-01. |
| Abstract | |
This study aimed to investigate whether the -1026(A>C)(rs2779249) and +2087(A>G)(2297518) polymorphisms in the NOS2 gene were associated with chronic periodontitis (CP) and with salivary levels of nitrite (NO2-) and/or nitrate + nitrite (NOx). A group of 113 mixed-race patients were subjected to periodontal, genetic, and biochemical evaluations (65 CP/48 periodontally healthy subjects). DNA was extracted from oral epithelial cells and used for genotyping by polymerase chain reaction (real-time). Salivary NOx concentrations were determined using an ozone-based chemiluminescence assay. Association of CP with alleles and genotypes of the -1026(A>C) polymorphism was found (X-2 test, p = 0.0075; 0.0308), but this was not maintained after multiple logistic regression, performed to estimate the effect of covariates and polymorphisms in CP. This analysis demonstrated, after correction for multiple comparisons, that only the female gender was significantly associated with CP. Polymorphisms analyzed as haplotypes were not associated with CP. NOx levels were significantly higher in the control group of heterozygous individuals for both polymorphisms. In conclusion, the female gender was significantly associated with CP, and higher levels of salivary NOx were found in control subjects and associated with the heterozygous state of the NOS2 polymorphisms, reinforcing the potential of NO metabolites as markers of periodontitis status. (AU) | |
| FAPESP's process: | 05/03231-7 - Investigation of polymorphisms in the promoter region of interleukin 8 in Brazilian individuals with chronic periodontitits |
| Grantee: | Yeon Jung Kim |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 03/10424-0 - Investigation of polymorphisms in the promoter region of interleukin 8 in Brazilian individuals with chronic periodontitits |
| Grantee: | Raquel Mantuaneli Scarel Caminaga |
| Support Opportunities: | Regular Research Grants |