| Full text | |
| Author(s): |
Cecchi, C. R.
;
Higuti, E.
;
Lima, E. R.
;
Vieira, D. P.
;
Squair, P. L.
;
Peroni, C. N.
;
Bartolini, P.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | CURRENT MOLECULAR MEDICINE; v. 17, n. 3, p. 6-pg., 2017-01-01. |
| Abstract | |
Background: An alternative treatment for growth hormone deficiency based on hGH-DNA administration, followed by electro gene transfer, was investigated by injecting the plasmid into surgically exposed or non-exposed quadriceps or tibialis muscle of immunodeficient "little" mice. Methods: An optimization of electrotransfer conditions via a new combination of high/low voltage pulses is presented. After 3 days, serum hGH was determined and in a 28-day assay, the relative growth parameters were compared. Results: Both groups exhibited similar results: 5.0 +/- 2.2 (SD) and 3.5 +/- 0.9 ng hGH/ml (P>0.05; n=7) for the exposed quadriceps and non-exposed tibialis treatments, respectively. The final body weight increases were 16.1% for the quadriceps and 18.9% for the tibialis group. The tail and nose-to-tail length increases were 4.5% and 7.1% for the quadriceps and 4.8 and 4.6% for the tibialis group. The right and left femur length increases, obtained from radiographic measurements, were 16.9% and 12.7% for the quadriceps and 19.4% and 12.3% for the tibialis, respectively. A non-significant difference between exposed quadriceps and non-exposed tibialis treatments (P=0.48) was confirmed via a completely integrated statistical analysis. Circulating mIGF-1 levels were 126 +/- 47, 106 +/- 93 (P>0.05) and 38 +/- 15 ng/ml for the quadriceps, tibialis and saline treatments, respectively. Conclusion: These results show that hGH-DNA administration into non-exposed tibialis muscle followed by the new HV/LV electrotransfer protocol was an equally efficient, less traumatic treatment, much more suitable for pre-clinical testing than administration into exposed quadriceps. (AU) | |
| FAPESP's process: | 13/03747-0 - Growth hormone gene therapy: homologous treatment with plasmid DNA and with implant of microencapsulated cells in immunocompetent dwarf mice (lit/lit) |
| Grantee: | Claudia Regina Cecchi |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/19757-7 - Growth hormone gene therapy: plasmid DNA injection and microencapsulated cells implantation in dwarf mice |
| Grantee: | Cibele Nunes Peroni |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/07380-6 - Optimization of in vivo growth hormone gene transfer parameters for phenotypic correction of dwarf mice |
| Grantee: | Eliana Rosa Lima Filha |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/04277-0 - Growth Hormone Gene therapy: homologous treatment based on plasmid DNA administration and on microencapsulated cells implanted in immunocompetent dwarf mice (lit/lit) |
| Grantee: | Claudia Regina Cecchi |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 14/18242-3 - Gene therapy for growth hormone deficiency mediated by minicircle DNA electrotransfer in dwarf mice |
| Grantee: | Eliza Higuti Sousa |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |