Activity of 3 '-hydroxychalcone against Cryptococcus gattii and toxicity, and efficacy in alternative animal models

Author(s):Show less -

Palanco, Ana Cerrejn ; Singulani, Junya de lacorte ; Costa-Orlandi, Caroline Barcelos ; Gullo, Fernanda Patricia ; Strohmayer Lourencetti, Natalia Manuela ; Gomes, Paulo Cesar ; Ayusso, Gabriela Miranda ; Dutra, Luiz Antonio ; Bolzani, Vanderlan da Silva ; Regasini, Luis Octavio ; Soares Mendes-Giannini, Maria Jose ; Fusco-Almeida, Ana Marisa

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 12, n. 13, p. 12-pg., 2017-10-01.

Abstract

Aim: This work aimed to evaluate the activity of 3'-hydroxychalcone against Cryptococcus gattii in planktonic and biofilm forms and their toxicity using alternative animal models. Materials & methods: Minimum inhibitory concentration and minimum fungicide concentration were determined. Biofilm formation and the susceptibility tests were performed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[carbonyl(phenylamino)]2H-tetrazolium hydroxide assay. Toxicity and efficacy were checked in Danio rerio and Galleria mellonella models. Results: The compound 3'-hydroxychalcone showed fungicidal activity against C. gattii in both planktonic and biofilm forms. The toxicity in zebrafish embryos revealed a low lethal concentration. In G. mellonella, the compound did not show antifungal activity and larvae toxicity. Conclusion: Because of the activity of 3'-hydroxychalcone against C. gattii in vitro, molecular modifications should be made to improve efficacy and to reduce toxicity in vivo. (AU)

• FAPESP's process: 14/10446-9 - Evaluation of new therapies and biomarkers in paracoccidioidomycosis: antifungal activity of alkyl gallates in alternative models and mice and identification of circulating microRNAs
• Grantee: Junya de Lacorte Singulani
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/18330-0 - Synthesis and biological evaluation of curcumin-cinnamaldehyde hybrids as bacterial cell division inhibitors
• Grantee: Luis Octávio Regasini
• Support Opportunities: Regular Research Grants