Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

Author(s)

Freitas, A. P. ; Favoretto, B. C. ; Clissa, P. B. ; Sampaio, S. C. ; Faquim-Mauro, E. L.

• Document type: Journal article
• Source: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2018, p. 15-pg., 2018-01-01.

Abstract

The Crotalus durissus terrificus rattlesnake venom, its main toxin, crotoxin (CTX), and its crotapotin (CA) and phospholipase A(2) (CB) subunits modulate the immune system. Formyl peptide receptors (FPRs) and lipoxin A(4) (LXA(4)) are involved in CTX's effect on macrophages and neutrophils. Dendritic cells (DCs) are plasticity cells involved in the induction of adaptive immunity and tolerance maintenance. Therefore, we evaluated the effect of CTX, CA or CB on the maturation of DCs derived from murine bone marrow (BM). According to data, CTX and CB-but not CA-induced an increase of MHC-II, but not costimulatory molecules on DCs. Furthermore, CTX and CB inhibited the expression of costimulatory and MHC-II molecules, secretion of proinflammatory cytokines and NF-kappa Bp65 and p38/ERK1/2-MAPK signaling pathways by LPS-incubated DCs. Differently, CTX and CB induced IL-10, PGE(2) and LXA(4) secretion in LPS-incubated DCs. Lower proliferation and IL-2 secretion were verified in coculture of CD3(+) cells and DCs incubated with LPS plus CTX or CB compared with LPS-incubated DCs. The effect of CTX and CB on DCs was abolished in cultures incubated with a FPRs antagonist. Hence, CTX and CB exert a modulation on functional activity of DCs; we also checked the involvement the FPR family on cell activities. (AU)

• FAPESP's process: 14/13085-7 - Molecular mechanisms involved in the suppression of the immune response to helminths: intracellular signaling pathways induced by the interaction of Ascaris suum antigens with DC-SIGN and MR on dendritic cells
• Grantee: Bruna Cristina Favoretto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/10393-1 - Study of the involvement of DC-SIGN and MR receptors in the mechanisms of immunesuppression induced by high molecular weight components of Ascaris suum extract
• Grantee: Bruna Cristina Favoretto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/15608-0 - Evaluation of the molecular mechanisms involved in the modulation of immune response by high molecular weight components of Ascaris suum, crotoxin and phospholipase A2 from Crotalus durissus terrificus venom
• Grantee: Eliana Faquim de Lima Mauro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23735-0 - Mechanisms involved in the modulation of the immune response induced by high molecular weight components of Ascaris suum extract and crotoxin isolated from Crotalus durissus terrificus venom
• Grantee: Eliana Faquim de Lima Mauro
• Support Opportunities: Regular Research Grants