| Full text | |
| Author(s): |
Reis, Luiza C.
;
Ramos-Sanchez, Eduardo Milton
;
Petitto-Assis, Fabricio
;
Nerland, Audun H.
;
Hernandez-Valladares, Maria
;
Selheim, Frode
;
Floeter-Winter, Lucile Maria
;
Goto, Hiro
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Mediators of Inflammation; v. 2018, p. 17-pg., 2018-01-01. |
| Abstract | |
Certain cytokines modulate the expression of insulin-like growth factor-(IGF-) I. Since IL-4 and IGF-I promote growth of the protozoan Leishmania major, we here addressed their interaction in downregulating the expression of Igf-I mRNA using small interfering RNA (siRNA) in Leishmania major-infected macrophages. Parasitism was decreased in the siRNA-treated cells compared with the nontreated cells, reversed by the addition of recombinant IGF-I (rIGF-I). In IL-4-stimulated macrophages, parasitism and the Igf-I mRNA amount were increased, and the effects were nullified upon siRNA transfection. IGF-I downregulation inhibited both parasite and macrophage arginase activation even in IL-4-stimulated cells. Searching for intracellular signaling components shared by IL-4 and IGF-I, upon siRNA transfection, phosphorylated p44, p38, and Akt proteins were decreased, affecting the phosphatidylinositol-3-kinase (PI3K)/Akt pathway. In L. major-infected C57BL6-resistant mice, the preincubation of the parasite with rIGF-I changed the infection profile to be similar to that of susceptible mice. We conclude that IGF-I constitutes an effector element of IL-4 involving the PI3K/Akt pathway during L. major infection. (AU) | |
| FAPESP's process: | 08/04106-0 - The role of insulin like-growth factor I (IGF-I) in the modulation of cytokines effect in resistance and susceptibility in murine cutaneous leishmaniasis infected by Leishmania (L.) major |
| Grantee: | Luiza de Campos Reis |
| Support Opportunities: | Scholarships in Brazil - Doctorate |