Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy

Author(s)

De Abreu, Dilayla K. ; Monteiro, Janaina M. ; Souza, Carolina C. ; Karam, Rafael G. ; Fernandes, Renata A. ; Lessa, Thais B. ; Fagliari, Jose J. ; Miglino, Maria Angelica ; Ambrosio, Carlos Eduardo

• Document type: Journal article
• Source: Anais da Academia Brasileira de Ciências; v. 90, n. 3, p. 14-pg., 2018-07-01.

Abstract

Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is consider the most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic's (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data. (AU)

• FAPESP's process: 07/51222-2 - Interaction of cell and gene therapy for the correction of Duchenne muscular dystrophy using chimeric endonucleases
• Grantee: Carlos Eduardo Ambrósio
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 09/02958-1 - Standardization of blood profile, biochemical, serum protein and immunophenotype of lymphocytes in healthy Golden Retriever and affected by muscular dystrophy.
• Grantee: Dilayla Kelly de Abreu
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/09392-9 - Production of induced pluripotent stem cells as model studies in rabbits
• Grantee: Carlos Eduardo Ambrósio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/13030-0 - In vitro Immunomodulation of mesenchymal stem cells in Golden Retriever healthy and affected dogs by muscular dystrophy.
• Grantee: Dilayla Kelly de Abreu
• Support Opportunities: Scholarships in Brazil - Doctorate