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CELL SEGMENTATION IN 3D CONFOCAL IMAGES USING SUPERVOXEL MERGE-FORESTS WITH CNN-BASED HYPOTHESIS SELECTION

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Author(s):
Stegmaier, Johannes ; Spina, Thiago V. ; Falcao, Alexandre X. ; Bartschat, Andreas ; Mikut, Ralf ; Meyerowitz, Elliot ; Cunha, Alexandre ; IEEE
Total Authors: 8
Document type: Journal article
Source: 2018 IEEE 15TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI 2018); v. N/A, p. 5-pg., 2018-01-01.
Abstract

Automated segmentation approaches are crucial to quantitatively analyze large-scale 3D microscopy images. Particularly in deep tissue regions, automatic methods still fail to provide error-free segmentations. To improve the segmentation quality throughout imaged samples, we present a new supervoxel-based 3D segmentation approach that outperforms current methods and reduces the manual correction effort. The algorithm consists of gentle preprocessing and a conservative super-voxel generation method followed by supervoxel agglomeration based on local signal properties and a postprocessing step to fix under-segmentation errors using a Convolutional Neural Network. We validate the functionality of the algorithm on manually labeled 3D confocal images of the plant Arabidopsis thaliana and compare the results to a state-of-the-art meristem segmentation algorithm. (AU)

FAPESP's process: 15/09446-7 - Medical Image Segmentation: How to integrate object appearance/shape models and interactive correction with minimum user intervention?
Grantee:Thiago Vallin Spina
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/11853-2 - SAMSAM: Segmentation for Analysis and Measurements in the Shoot Apical Meristem
Grantee:Thiago Vallin Spina
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 14/12236-1 - AnImaLS: Annotation of Images in Large Scale: what can machines and specialists learn from interaction?
Grantee:Alexandre Xavier Falcão
Support Opportunities: Research Projects - Thematic Grants