| Full text | |
| Author(s): Show less - |
Pathak, Monika
;
Manna, Rosa
;
Li, Chan
;
Kaira, Bubacarr G.
;
Hamad, Badraldin Kareem
;
Belviso, Benny Danilo
;
Bonturi, Camila R.
;
Dreveny, Ingrid
;
Fischer, Peter M.
;
Dekker, Lodewijk V.
;
Vilela Oliva, Maria Luiza
;
Emsley, Jonas
Total Authors: 12
|
| Document type: | Journal article |
| Source: | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY; v. 75, p. 14-pg., 2019-06-01. |
| Abstract | |
Coagulation factor XII (FXII) is a key initiator of the contact pathway, which contributes to inflammatory pathways. FXII circulates as a zymogen, which when auto-activated forms factor XIIa (FXIIa). Here, the production of the recombinant FXIIa protease domain (FXIIa(His)) with yields of approximate to 1-2mg per litre of insect-cell culture is reported. A second construct utilized an N-terminal maltose-binding protein (MBP) fusion (MBP-FXIIa(His)). Crystal structures were determined of MBP-FXIIa(His) in complex with the inhibitor d-Phe-Pro-Arg chloromethyl ketone (PPACK) and of FXIIa(His) in isolation. The FXIIa(His) structure revealed that the S2 and S1 pockets were occupied by Thr and Arg residues, respectively, from an adjacent molecule in the crystal. The Thr-Arg sequence mimics the P2-P1 FXIIa cleavage-site residues present in the natural substrates prekallikrein and FXII, and Pro-Arg (from PPACK) mimics the factor XI cleavage site. A comparison of the FXIIa(His) structure with the available crystal structure of the zymogen-like FXII protease revealed large conformational changes centred around the S1 pocket and an alternate conformation for the 99-loop, Tyr99 and the S2 pocket. Further comparison with activated protease structures of factors IXa and Xa, which also have the Tyr99 residue, reveals that a more open form of the S2 pocket only occurs in the presence of a substrate mimetic. The FXIIa inhibitors EcTI and infestin-4 have Pro-Arg and Phe-Arg P2-P1 sequences, respectively, and the interactions that these inhibitors make with FXIIa are also described. These structural studies of FXIIa provide insight into substrate and inhibitor recognition and establish a scaffold for the structure-guided drug design of novel antithrombotic and anti-inflammatory agents. (AU) | |
| FAPESP's process: | 17/06630-7 - Fragments derived from the structure of protease inhibitors with selectivity for inhibition of mammalian and microorganism enzymes and its role as an anti-inflammatory, antimicrobial, antithrombotic and anti- tumor agent: mechanism of action |
| Grantee: | Maria Luiza Vilela Oliva |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/07972-9 - Development of lead agents for prophylaxis and treatment of cardiovascular diseases |
| Grantee: | Maria Luiza Vilela Oliva |
| Support Opportunities: | Regular Research Grants |