Light excess stimulates Poly-beta-hydroxybutyrate yield in a mangrove-isolated strain of Synechocystis sp.

Author(s)

Gracioso, Louise Hase ; Bellan, Alessandra ; Karolski, Bruno ; Bispo Cardoso, Leticia Oliveira ; Perpetuo, Elen Aquino ; Oller do Nascimento, Claudio Augusto ; Giudici, Reinaldo ; Pizzocchero, Valentino ; Basaglia, Marina ; Morosinotto, Tomas

• Document type: Journal article
• Source: Bioresource Technology; v. 320, p. 7-pg., 2021-01-01.

Abstract

Poly-beta-hydroxybutyrate (PHB) is a biodegradable biopolymer that may replace fossil-based plastics reducing its negative environmental impact. One highly sustainable strategy to produce these biopolymers is the exploitation of photosynthetic microorganisms that use sunlight and CO2 to produce biomass and subsequently, PHB. Exploring environmental biological diversity is a powerful tool to find resilient microorganisms potentially exploitable to produce bioproducts. In this work, a cyanobacterium (Synechocystis sp.) isolated from a contaminated area close to an important industrial complex was shown to produce PHB under different culture conditions. Carbon, nutrients supply and light intensity impact on biomass and PHB productivity were assessed, showing that the highest yield of PHB achieved was 241 mg L-1 (31%dcw) under high light intensity. Remarkably this condition not only stimulated PHB accumulation by 70% compared to other conditions tested but also high cellular duplication rate, maximizing the potential of this strain for PHB production. (AU)

• FAPESP's process: 17/17438-0 - Mitigation of CO2 and CH4 using microbial consortium (algae and bacteria)
• Grantee: Louise Hase Gracioso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/10811-0 - Optimization of growing conditions to induce production of high value molecules from algae
• Grantee: Louise Hase Gracioso
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/50279-4 - Brasil Research Centre for Gas Innovation
• Grantee: Julio Romano Meneghini
• Support Opportunities: Research Grants - Research Centers in Engineering Program
• FAPESP's process: 18/22790-7 - Evaluation of the potential of the production of biopolymers by methylotrophic bacteria
• Grantee: Elen Aquino Perpetuo
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants