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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Further characterization of the subunits of the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) by SDS-PAGE electrophoresis and MALDI-TOF-MS

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Author(s):
Carvalho, Francisco Adriano O. [1] ; Carvalho, Jose Wilson P. [1] ; Santiago, Patricia S. [1] ; Tabak, Marcel [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Process Biochemistry; v. 46, n. 11, p. 2144-2151, NOV 2011.
Web of Science Citations: 17
Abstract

Further characterization of hemoglobin of Glossoscolex paulistus (HbGp) subunits was performed based on SDS-PAGE, size exclusion chromatography (SEC) and MALDI-TOF-MS analysis. SDS-PAGE has shown a total of four linker chains, two quite intense and two of lower intensity. HbGp fractions (I-VI), obtained by size exclusion chromatography (SEC), from oligomeric dissociation at alkaline pH 9.6, were monitored. Fraction 1 is identical to the whole protein. The monomeric chains c, obtained from the trimer abc reduction, present four isoforms with MM 17,336 Da, 17,414 Da, 17,546 Da and 17,620 Da. Furthermore, the trimer subunit presents two isoforms, T(1) and T(2), with MM 51,200 +/- 60 and 51,985 +/- 50 Da, respectively. Based on SDS-PAGE, the linker chains seem to be distributed along the different fractions of the SEC chromatogram, appearing along the peaks corresponding to fractions I-V. The fraction IV contains, predominantly, trimers with some linkers contamination. The strong interaction of linker chains L with the trimers abc, makes it difficult to obtain these subunits in pure form. The monomer d in fraction VI appears to be quite pure, in agreement with previous studies. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/17261-6 - Oligomeric stability studies of giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) in the presence of chaotropic agents, surfactants and characterization of its subunits.
Grantee:Francisco Adriano de Oliveira Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/09719-0 - Effects of surfactants, iron oxidation state and medium pH upon the thermal stability of giant extracellular hemoglobin of Glossoscolex paulistus in its native oligomeric form and for trimeric ABC and monomeric d globin subunits
Grantee:José Wilson Pires Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate