The crosstalk between bone metabolism, lncRNAs, microRNAs and mRNAs in coronary artery calcification

Author(s)

Wicik, Zofia ; Jales Neto, Levi H. ; Guzman, Luis E. F. ; Pavao, Rodrigo ; Takayama, Liliam ; Caparbo, Valeria F. ; Lopes, Neuza H. M. ; Pereira, Alexandre C. ; Pereira, Rosa M. R.

• Document type: Journal article
• Source: Genomics; v. 113, n. 1, p. 11-pg., 2021-01-25.

Abstract

The association between Coronary Artery Calcification (CAC) and osteoporosis has been reported but not fully understood. Therefore, using an original bioinformatic framework we analyzed transcriptomic profiles of 20 elderly women with high CAC score and 31 ageand sex-matching controls from Sao Paulo Ageing & Health study (SPAH). We integrated differentially expressed microRNA (miRNA) and long-noncoding RNA (lncRNA) interactions with coding genes associated with CAC, in the context of bone-metabolism genes mined from literature. Top non-coding regulators of bone metabolism in CAC included miRNA 497-5p/195 and 106a-5p, and lncRNA FAM197Y7. Top non-coding RNAs revealed significant interplay between genes regulating bone metabolism, vascularization-related processes, chromatin organization, prostaglandin and calcium co-signaling. Prostaglandin E2 receptor 3 (PTGER3), Fibroblasts Growth Factor Receptor 1 (FGFR1), and One Cut Homeobox 2 (ONECUT2) were identified as the most susceptible to regulation by the top non-coding RNAs. This study provides a flexible transcriptomic framework including non-coding regulation for biomarker-related studies. (AU)

• FAPESP's process: 18/07135-2 - Evaluation of Bone Metabolism Parameters by HR-pQCT and Histomorphometry in Rheumatoid Arthritis and Juvenile Idiopathic Arthritis Patients: Association with Clinical and Genetic Variables
• Grantee: Zofia Agnieszka Wicik
• Support Opportunities: Scholarships in Brazil - Post-Doctoral