The role of annexin A1 in Candida albicans and Candida auris infections in murine neutrophils

Author(s)

Sanches, Jose Marcos ; Rossato, Luana ; Lice, Izabella ; Alves de Piloto Fernandes, Anna Maria ; Bueno Duarte, Gustavo Henrique ; Rosini Silva, Alex Aparecido ; Porcari, Andreia de Melo ; Carvalho, Patricia de Oliveira ; Gil, Cristiane Damas

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 150, p. 8-pg., 2021-01-01.

Abstract

Annexin A1 (AnxA1) is an anti-inflammatory protein expressed in various cell types, especially macrophages and neutrophils. Because neutrophils play important roles in infections and inflammatory processes and the relationship between AnxA1 and Candida spp. infections is not well-understood, our study examined whether AnxA1 can serve as a target protein for the regulation of the immune response during fungal infections. C57BL/6 wild-type (WT) and AnxA1 knockout (AnxA1(-/)(-)) peritoneal neutrophils were coinfected with Candida albicans or Candida auris for 4 h. AnxA1(-/-) neutrophils exhibited a marked increase in cyclooxygenase 2 (COX-2), phosphorylated extracellular signal-related kinase (ERK), p-38, and c-Jun N-terminal kinase (JNK) levels after coinfection with both Candida spp. A lipidomics approach showed that AnxA1 deficiency produced marked differences in the supernatant lipid profiles of both control neutrophils and neutrophils coinfected with Candida spp. compared with WT cells, especially the levels of glycerophospholipids and glycerolipids. Our results showed that endogenous AnxA1 regulates the neutrophil response under fungal infection conditions, altering lipid membrane organization and metabolism. (AU)

• FAPESP's process: 20/03565-2 - Effect of annexin A1 and its mimetic peptides in models of inflammatory response in vitro (2D and 3D) and acute toxicity in vivo
• Grantee: Cristiane Damas Gil
• Support Opportunities: Regular Research Grants