Adjuvant ArtinM favored the host immunity against Cryptococcus gattii infection in C57BL/6 mice

Author(s)

Oliveira-Brito, Patricia Kellen Martins ; de Campos, Gabriela Yamazaki ; Guimaraes, Julia Garcia ; Machado, Michele Procopio ; Serafim, Leticia Costa ; Chica, Javier Emilio Lazo ; Roque-Barreira, Maria Cristina ; da Silva, Thiago Aparecido

• Document type: Journal article
• Source: Immunotherapy; v. 16, n. 11, p. 16-pg., 2024-06-20.

Abstract

Aim: Cryptococcus gattii causes a severe fungal infection with high mortality rate among immunosuppressed and immunocompetent individuals. Due to limitation of current antifungal treatment, new immunotherapeutic approaches are explored. Methods: This study investigated an immunization strategy utilizing heat-inactivated C. gattii with ArtinM as an adjuvant. C57BL/6 mice were intranasally immunized with heat-killed C. gattii and ArtinM was administrated either before immunization or along with HK-C. gattii. Mice were infected with C. gattii and the efficacy of the immunization protocol was evaluated. Results: Mice that received ArtinM exhibited increased levels of IL-10 and relative expression of IL-23 in the lungs, reduced fungal burden and preserved tissue integrity post-infection. Conclusion: Adjuvant ArtinM improved immunization against C. gattii infection in C57BL/6 mice. (AU)

• FAPESP's process: 18/18538-0 - Bioengineered of T- and NK-cells by CAR against invasive fungal infections
• Grantee: Thiago Aparecido da Silva
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/09260-1 - Dectin-1 ligands as immunostimulant agents in a vaccination strategy against experimental cryptococcosis
• Grantee: Letícia Serafim da Costa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/26074-7 - Bioengineered of T- and NK-cells by CAR against invasive fungal infections
• Grantee: Thiago Aparecido da Silva
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 19/09261-8 - Immunotherapy against experimental cryptococcosis: a strategy of vaccination with TLR2 agonists as immunostimulating agents
• Grantee: Júlia Garcia Guimarães
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/19949-4 - Immunotherapy against experimental Cryptococcosis: a vaccine strategy associated with immunostimulators that recognize carbohydrate
• Grantee: Patrícia Kellen Martins Oliveira Brito
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis
• Grantee: Thiago Aparecido da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/21708-5 - Application of immunomoduladors, by carbohydrate recognition, as therapeutic agents: mechanism of action to immunotherapy
• Grantee: Maria Cristina Roque Antunes Barreira
• Support Opportunities: Research Projects - Thematic Grants