Backbone and sidechain NMR assignments of residues 1-81 from yeast Sis1 in complex with an Hsp70 C-terminal EEVD peptide

Author(s)

Matos, Carolina O. ; Pinheiro, Glaucia ; Ramos, Carlos ; Almeida, Fabio

• Document type: Journal article
• Source: BIOMOLECULAR NMR ASSIGNMENTS; v. 17, n. 2, p. 4-pg., 2023-08-17.

Abstract

Molecular chaperones aid proteins to fold and assemble without modifying their final structure, requiring, in several folding processes, the interplay between members of the Hsp70 and Hsp40 families. Here, we report the NMR chemical shift assignments for (1) H, (15) N, and (13) C nuclei of the backbone and side chains of the J-domain of the class B Hsp40 from Saccharomyces cerevisiae, Sis1, complexed with the C-terminal EEVD motif of Hsp70. The data revealed information on the structure and backbone dynamics that add significantly to the understanding of the J-domain-Hsp70-EEVD mechanism of interaction. (AU)

• FAPESP's process: 19/16114-1 - Structure / function relationship of the Hsp40s.
• Grantee: Carolina Oliveira Matos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/01074-9 - HSP90 interaction with other proteins and cofactors
• Grantee: Glaucia Melina Squizato Pinheiro de Castro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/26131-5 - The chaperome: study of the relationship of the structure of its components and the maintenance of proteostasis
• Grantee: Carlos Henrique Inacio Ramos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/11948-9 - Interaction mechanism between the co-chaperone J-domain protein with their substrates and the chaperone Hsp70
• Grantee: Glaucia Melina Squizato Pinheiro de Castro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/50161-8 - Study of the structure and function of the Hsp90 chaperone with emphasis on its role in cellular homeostasis
• Grantee: Carlos Henrique Inacio Ramos
• Support Opportunities: Research Projects - Thematic Grants