hsa-miR-582-3p in umbilical cord blood is negatively associated with maternal exposure to childhood maltreatment

Author(s):Show less -

Micali, Danilo ; Rosa, Joice Santos ; Ota, Vanessa Kiyomi ; Mercuri, Rafael Luiz Vieira ; Spindola, Leticia Maria ; Bugiga, Amanda Victoria Gomes ; Kajitani, Gustavo Satoru ; Milani, Ana Carolina Coelho ; Salmeron, Camilla ; Silva, Ivaldo ; Asprino, Paula Fontes ; Galante, Pedro A. F. ; Duarte, Cristiane Seixas ; Posner, Jonathan ; Jackowski, Andrea Parolin ; Belangero, Sintia Iole

• Document type: Journal article
• Source: Epigenomics; v. N/A, p. 14-pg., 2024-10-09.

Abstract

Aim: Childhood maltreatment (CM) may affect not only directly exposed individuals but also their offspring. However, the underlying biological mechanisms remain unclear. microRNAs (miRNAs) may play a regulatory role in this process. This study investigates the relationship between maternal exposure to CM and miRNA expression in maternal and perinatal tissues. Methods: We enrolled 43 pregnant women and assessed their CM exposure. We collected maternal blood, cord blood and placental tissue samples during childbirth and performed miRNA profiling using next generation sequencing. Results: Maternal CM was inversely associated with hsa-miR-582-3p levels in cord blood. Pathway analysis revealed that this miRNA regulates genes involved in intrauterine development. Conclusion: Our findings highlight the potential impact of maternal CM exposure on offspring epigenetic mechanisms. PLAIN LANGUAGE SUMMARY Child maltreatment (CM) includes physical, sexual and emotional abuse, as well as physical and emotional neglect. CM not only harms those directly exposed but can also negatively impact their offspring. However, the biological reasons behind this are not well understood. To explore this further, our study investigates how CM affects the biology of pregnant women and their newborns through changes in small regulatory molecules called microRNAs (miRNAs). We recruited 43 pregnant women and assessed their exposure to CM. During childbirth, we collected blood samples from the mothers, blood from the umbilical cord and placental samples. We then analyzed the levels of miRNAs in these samples using advanced sequencing technology. We observed that more severe maternal exposure to CM was associated with lower levels of a miRNA named hsa-miR-582-3p in umbilical cord blood. This miRNA regulates genes involved in fetal development in utero and has been linked to spontaneous preterm birth. It may also influence immunologic and stress-related processes. Thus, newborns of mothers who had been exposed to CM may be more vulnerable to adverse effects on their brain development and overall health. Despite our small sample size, our study highlights the importance of addressing CM as an intergenerational concern and provides new insights into the biological mechanisms through which maternal CM can affect offspring. (AU)

• FAPESP's process: 19/21612-0 - Maternal adversity, inflammation, and neurodevelopment: how intergenerational processes perpetuate disadvantage in a low-resource setting
• Grantee: Andrea Parolin Jackowski
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/15579-8 - Retroelements: a driving force generating genetic novelties in the human and mouse genome
• Grantee: Pedro Alexandre Favoretto Galante
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/02413-4 - Intragenic retrocopies as a source of novel protein domains in humans
• Grantee: Rafael Luiz Vieira Mercuri
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)