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A conductive film produced by the supernatant from Serratia marcescens cultivation containing prodigiosin increases electricity generation in a microbial fuel cell

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Author(s):
Zani, Ana Clara Bonizol ; de Souza, Joao Carlos ; Furlan, Joao Pedro Rueda ; Stehling, Eliana Guedes ; de Andrade, Adalgisa Rodrigues ; Reginatto, Valeria
Total Authors: 6
Document type: Journal article
Source: CURRENT RESEARCH IN BIOTECHNOLOGY; v. 7, p. 11-pg., 2024-04-24.
Abstract

Although Serratia marcescens is known for its natural ability to produce the red pigment prodigiosin, it has been little explored as a biocatalyst in bioelectrochemical systems (BES). Here, we have employed an environmental S. marcescens isolate S734 as biocatalyst in a microbial fuel cell (MFC) anode to oxidize glycerol and to produce energy; we have evaluated how the anode behaves in three conditions: (i) as an abiotic electrode (FC-A); (ii) as a biotic electrode after S. marcescens biofilm growth (MFC-B); and (iii) as an abiotic electrode added with the supernatant containing prodigiosin (FC-P). Scanning electron microscopy and electrochemical measurements indicated that prodigiosin formed a conductive film over FC-P, which increased charge transfer by 424 times compared to FC-A. The maximum power density during the FC-P operation was 10.0 mW/m(-2). Nevertheless, only in the presence of S. marcescens (MFC-B) was glycerol oxidized and electricity generated. Cyclic voltammetry indicated that the prodigiosin was the electrochemical active substance in the supernatant, and that its process was irreversible and controlled by adsorption. Electrochemical impedance spectroscopy confirmed that the prodigiosin-containing supernatant decreased the load resistance from 8396.3 ohm in FC-A to 58.10 ohm in FC-P. Genomic analysis showed that the prodigiosin biosynthesis gene cluster in strain S734 belonged to the Serratia 274 type, which contains pigA to pigN genes flanked by cueR and copA homologues. In conclusion, the supernatant produced by S. marcescens strain S734, containing prodigiosin could be explored as a green conductor in BES without further purification steps. (AU)

FAPESP's process: 21/01655-7 - Resistome, plasmidome and virulome of Enterobacterales isolated from the environment carrying mcr-like genes
Grantee:Eliana Guedes Stehling
Support Opportunities: Regular Research Grants
FAPESP's process: 21/07294-6 - Contribution of Pseudomonas aeruginosa strains to bioelectrochemicals systems for obtaining energy and products from glycerol
Grantee:Ana Clara Bonizol Zani
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 22/04024-0 - Cofermentation of organic and inorganic residues in biological and bioelectrochemical systems for obtaining bioproducts and electricity
Grantee:Valeria Reginatto Spiller
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 21/12866-9 - Development of electrochemical sensors used in the electrochemical detection of BTEXs in petroleum wastewater self-fed by a microbial fuel cell: BTEXs detection, treatment and energy production
Grantee:João Carlos de Souza
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 21/01134-7 - Electrochemical valorization of glyceol molecule: development of a microbial electrolytic cell (MEC) to produce bio-hydrogen (Bio-H2) and value add products in a pem fuel cell
Grantee:Adalgisa Rodrigues de Andrade
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants