Maternal protein restriction combined with postnatal sugar consumption alters liver proteomic profile and metabolic pathways in adult male offspring rats

Author(s):Show less -

Ribeiro, Isabelle Tenori ; Fioretto, Matheus Naia ; dos Santos, Sergio Alexandre Alcantara ; Colombelli, Ketlin Thassiani ; Portela, Luis Marcos Frediani ; Alvarez, Marcus Vinicius Nis ; de Magalhaes Padilha, Pedro ; Delgado, Aislan Quintiliano ; Marques, Marcus Vinicius Lage Silva Giaculi ; Bosqueiro, Jose Roberto ; Seiva, Fabio Rodrigues Ferreira ; Barbisan, Luis Fernando ; de Andrade Paes, Antonio Marcus ; Zambrano, Elena ; Justulin Jr, Luis Antonio

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 592, p. 10-pg., 2024-06-20.

Abstract

This study investigated the impact of maternal protein restriction (MPR) and early postnatal sugar consumption (SUG) on the liver health of adult male descendant rats. Male offspring of mothers fed a normal protein diet (NPD) or a low protein diet (LPD) were divided into four groups: Control (CTR), Sugar Control (CTR + SUG), LPD during gestation and lactation (GLLP), and LPD with sugar (GLLP + SUG). Sugar consumption (10% glucose diluted in water) began after weaning on day 21 (PND 21), and at 90 days (PND 90), rats were sacrificed for analysis. Sugar intake reduced food intake and increased water consumption in CTR + SUG and GLLP + SUG compared to CTR and GLLP. GLLP and GLLP + SUG groups showed lower body weight and total and retroperitoneal fat compared to CTR and CTR + SUG. CTR + SUG and GLLP + SUG groups exhibited hepatocyte vacuolization associated with increased hepatic glycogen content compared to CTR and GLLP. Hepatic catalase activity increased in GLLP compared to CTR. Proteomic analysis identified 223 differentially expressed proteins (DEPs) among experimental groups. While in the GLLP group, the DEPs enriched molecular pathways related to cellular stress, glycogen metabolic pathways were enriched in the GLLP + SUG and CTR + SUG groups. The association of sugar consumption amplifies the effects of MPR, deregulating molecular mechanisms related to metabolism and the antioxidant system. (AU)

• FAPESP's process: 17/01063-7 - Integrative global analyzes of the rat ventral prostate submitted to maternal protein restriction and its repercussions with aging
• Grantee: Luis Antonio Justulin Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/01952-1 - Maternal protein restriction associated with post-weaning sugar consumption in offspring: Effects on the proteomic profile in the liver of young and senile rats
• Grantee: Isabelle Tenori Ribeiro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 22/03990-0 - Global expression profile of lncRNAs in the ventral prostate of offspring rats submitted to maternal low protein diet: a DOHaD approach
• Grantee: Luis Antonio Justulin Junior
• Support Opportunities: Regular Research Grants