| Full text | |
| Author(s): |
Silva, Mara Viana
;
Valente, Roniele Santana
;
Annes, Kelly
;
Marsico, Thamiris Vieira
;
Oliveira, Andressa Minozzo
;
Maiollo, Bruna Aparecida Prado
;
Lopes, Nelyane Jose
;
Tannura, Juliana Hayashi
;
Sudano, Mateus Jose
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 215, p. 7-pg., 2023-12-09. |
| Abstract | |
In vitro-produced embryos are constantly exposed to stressful conditions that can lead to the activation of the apoptotic pathway. The nuclear Kappa B factor (NF-kappa B) is an inflammatory mediator that induces the expression of tumor necrosis factor (TNF-alpha), a pro-inflammatory cytokine, while interleukin-10 (IL-10), an anti-inflammatory cytokine, inhibits NF-kappa B activity. This study aimed to investigate the effects of IL-10 and TNF-alpha on the competence and cryosurvival of in vitro-produced bovine embryos. Embryos were produced in vitro using standard protocols, and Grade I blastocysts were vitrified using the Cryotop method. Non-vitrified and vitrified blastocysts were subjected to the TUNEL assay. In Experiment I, on day 6.5 (156 h post-insemination), the embryos were treated with PBS (control), 50 ng/mL of IL-10, or a combination of 25 ng/mL of TNF-alpha and 50 ng/mL of IL-10. Embryonic development and apoptotic rates were monitored. In Experiment II, the same groups were set up, with the addition of a group treated with 25 ng/mL of TNF-alpha alone. Grade I blastocysts were vitrified 5 h after treatment, and cryosurvival was monitored at until 48 h post-warming. The apoptosis rate and total cell number were investigated in the vitrified-hatched blastocysts. IL-10 alone did not affect developmental competence or cryosurvival (P > 0.05). The IL-10-treated embryos, when exposed in combination with TNF-alpha, presented a detrimental effect (P < 0.05) in the embryonic development of non-vitrified embryos. However, vitrified blastocysts had no negative effect (P > 0.05). The TNF-alpha treatment reduced (P < 0.05) the re-expansion rate at 6 h post-warming and increased (P < 0.05) the apoptosis rate in vitrified hatched blastocysts, whereas no effect (P > 0.05) of the treatments was detected in the hatching rate and total cell number post-warming. In conclusion, TNF-alpha has a detrimental effect on embryonic developmental competence and cryosurvival by compromising the development of non-vitrified embryos and apoptotic-related events of vitrified blastocysts, whereas IL-10, when in combination with TNF-alpha, appears to attenuate the detrimental effects of TNF-alpha. (AU) | |
| FAPESP's process: | 21/01446-9 - Multi-user Equipment approved in grant 18/21496-8: Inverted Microscope Equipped with Fluorescence and Differential interference Contrast (DIC) |
| Grantee: | Mateus José Sudano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 19/17796-9 - Simultaneous biopsy and lipid profile of embryo inner cell mass and trofectoderm |
| Grantee: | Thamiris Vieira Marsico |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/21496-8 - Pathways for embryo-derived pregnancy |
| Grantee: | Mateus José Sudano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 20/08826-9 - Investigation of the cell membrane system and its relationship with cryotolerance and viability of bovine embryos produced in vitro |
| Grantee: | Kelly Annes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |