| Full text | |
| Author(s): |
Dantas, Adriane M. C.
;
Teixeira, Fernanda S.
;
Oblitas, Raissa L.
;
Araujo, Wagner W. R.
;
Amaro, Monique C.
;
Cajas, Rayssa A.
;
de Moraes, Josue
;
Salvadori, Maria C.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 14, n. 1, p. 12-pg., 2024-10-04. |
| Abstract | |
Schistosoma mansoni, an intravascular parasitic worm and the causative agent of schistosomiasis, relies on its tegument (outer layer) for survival and host interaction. This study explored the morphology and mechanical properties of S. mansoni tegument using Atomic Force Microscopy (AFM). Notably, we employed the PeakForce Quantitative Nanomechanical Mapping (PF-QNM) mode in air, enabling simultaneous acquisition of 3D topography and mechanical property contrasts (adhesion, elastic modulus). Additionally, nanoindentation (AFM contact mode) was performed on female worm tegument for elastic modulus measurement. Both techniques revealed an elastic modulus range of fractions or units of GPa for the tegument. Interestingly, mechanical property maps, particularly adhesion contrast, displayed a recurring pattern of light and dark bands. We also measured the depth of annular furrows on the female tegument, finding an average of 128 +/- 10 nm. These findings establish AFM, particularly PF-QNM, as a valuable tool to characterize S. mansoni tegument properties, offering insights for future investigations into parasite biology and its response to immunological or pharmacological challenges. (AU) | |
| FAPESP's process: | 23/08418-6 - Searching for bioactive metabolites from Brazilian biodiversity as drug candidates for diseases caused by parasitic worms |
| Grantee: | Josué de Moraes |
| Support Opportunities: | Regular Research Grants |