| Full text | |
| Author(s): |
Silva, Erica C.
;
Quinde, Carlos A.
;
Cieza, Basilio
;
Basu, Aakash
;
Vila, Marta M. D. C.
;
Balcao, Victor M.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | GENES; v. 15, n. 1, p. 35-pg., 2024-01-01. |
| Abstract | |
Coffee plants have been targeted by a devastating bacterial disease, a condition known as bacterial blight, caused by the phytopathogen Pseudomonas syringae pv. garcae (Psg). Conventional treatments of coffee plantations affected by the disease involve frequent spraying with copper- and kasugamycin-derived compounds, but they are both highly toxic to the environment and stimulate the appearance of bacterial resistance. Herein, we report the molecular characterization and mechanical features of the genome of two newly isolated (putative polyvalent) lytic phages for Psg. The isolated phages belong to class Caudoviricetes and present a myovirus-like morphotype belonging to the genuses Tequatrovirus (PsgM02F) and Phapecoctavirus (PsgM04F) of the subfamilies Straboviridae (PsgM02F) and Stephanstirmvirinae (PsgM04F), according to recent bacterial viruses' taxonomy, based on their complete genome sequences. The 165,282 bp (PsgM02F) and 151,205 bp (PsgM04F) genomes do not feature any lysogenic-related (integrase) genes and, hence, can safely be assumed to follow a lytic lifestyle. While phage PsgM02F produced a morphogenesis yield of 124 virions per host cell, phage PsgM04F produced only 12 virions per host cell, indicating that they replicate well in Psg with a 50 min latency period. Genome mechanical analyses established a relationship between genome bendability and virion morphogenesis yield within infected host cells. (AU) | |
| FAPESP's process: | 22/10775-9 - PsgPhageKill: isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae pv. garcae to fight the coffee bacterial canker: an efficient and ecofriendly alternative |
| Grantee: | Vitor Manuel Cardoso Figueiredo Balcão |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative |
| Grantee: | Vitor Manuel Cardoso Figueiredo Balcão |
| Support Opportunities: | Scholarships abroad - Research |