Point-of-Care Electrochemical Immunosensor Applied against Nosocomial Infection: Staphylococcus aureus Detection in Human Hand Skin

Author(s)

de Godoy, Henri Alves ; Faria, Aline Macedo ; Roza, Noemi Angelica Vieira ; Bach-Toledo, Larissa ; Simabuco, Fernando Moreira ; Scharlack, Nayara Kastem ; de Oliveira, Rodrigo Bueno ; Antunes, Adriane Elisabete Costa ; Arthur, Rangel ; Mazon, Talita

• Document type: Journal article
• Source: ACS INFECTIOUS DISEASES; v. 10, n. 6, p. 9-pg., 2024-05-14.

Abstract

Staphylococcus aureus is an important pathogen that causes nosocomial infections, resulting in unacceptable morbidity and mortality rates. In this work, we proposed the construction of a nanostructured ZnO-based electrochemical immunosensor for qualitative and semiquantitative detection of S. aureus using simple methods for growing zinc oxide nanorods (ZnO NRs) on a sensor board and immobilizing the anti-S. aureus antibody on ZnO NRs through cystamine and glutaraldehyde. The immunosensor detected S. aureus in the 10(3)-10(7) colony-forming unit (CFU) mL(-1) range and showed a limit of detection (LoD) around 0.792 x 10(3) CFU mL(-1). Beyond a satisfactory LoD, the developed immunosensor presented other advantages, such as high versatility for point-of-care assays and a suitable selective factor that admits the detection of the S. aureus concentration range in human hand skin after washing. Moreover, the immunosensor showed the potential to be an excellent device to control nosocomial infection by detecting the presence of S. aureus in human hand skin. (AU)

• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC