Neutral Red Film Augments Extracellular Electron Transfer Performed by Clostridium pasteurianum DSM 525

Author(s)

Zani, Ana Clara Bonizol ; de Souza, Joao Carlos ; de Andrade, Adalgisa Rodrigues ; Reginatto, Valeria

• Document type: Journal article
• Source: FERMENTATION-BASEL; v. 10, n. 10, p. 11-pg., 2024-10-01.

Abstract

Extracellular electron transfer (EET) is key to the success of microbial fuel cells (MFCs). Clostridium sp. often occurs in MFC anode communities, but its ability to perform EET remains controversial. We have employed Clostridium pasteurianum DSM 525 as a biocatalyst in a glycerol-fed MFC, designated MFCDSM. We have also followed the EET of this biocatalyst in the presence of a mediator, namely soluble neutral red (NR), soluble methyl viologen (MV), neutral red film (FNR), or methyl viologen film (FMV). MFCDSM provided power and current densities (j) of 0.39 mu W<middle dot>cm-2 and 2.47 mu A<middle dot>cm-2, respectively, which evidenced that the biocatalyst performs direct electron transfer (DET). Introducing 150.0 mu M NR or MV into the MFCDSM improved the current density by 7.0- and 3.7-fold (17.05 and 8.45 mu A<middle dot>cm-2), respectively. After 20 cyclic voltammetry (CV) cycles, the presence of FNR in the MFCDSM anodic chamber provided an almost twofold higher current density (30.76 mu A<middle dot>cm-2) compared to the presence of NR in the MFCDSM. Introducing MV or FMV into the MFCDSM anodic chamber gave practically the same current density after 10 CV cycles. The MFCDSM anodic electrode might interact with FMV weakly than with FNR, so FNR is more promising to enhance C. pasteurianum DSM 525 EET within MFCDSM. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/04024-0 - Cofermentation of organic and inorganic residues in biological and bioelectrochemical systems for obtaining bioproducts and electricity
• Grantee: Valeria Reginatto Spiller
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 21/12866-9 - Development of electrochemical sensors used in the electrochemical detection of BTEXs in petroleum wastewater self-fed by a microbial fuel cell: BTEXs detection, treatment and energy production
• Grantee: João Carlos de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 24/00725-0 - Environmentally friendly strategies for reducing carbon dioxide in value-added products through bioelectrochemical and photoelectrochemical pathways
• Grantee: Ana Clara Bonizol Zani
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 23/07992-0 - Towards Zero Impact Agriculture: bio-NAno architecTUres for ammonia REcovery - NATURE
• Grantee: Adalgisa Rodrigues de Andrade
• Support Opportunities: Regular Research Grants