Extracellular vesicles derived from bovine adipose-derived mesenchymal stromal cells enhance in vitro embryo production from lesioned ovaries

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Barcelos, Stefhani Martins ; Rosa, Paola Maria da Silva ; Moura, Ana Beatriz Bossois ; Villarroel, Carla Lujan Pereira ; Bridi, Alessandra ; Bispo, Elizabete Cristina Iseke ; Garcez, Emanuella Melgaco ; Oliveira, Gabriela de Souza ; Almeida, Maria Alice ; Malard, Patricia Furtado ; Peixer, Mauricio Antonio Silva ; Pereira, Rinaldo Wellerson ; Alencar, sergio Amorim de ; Saldanha-Araujo, Felipe ; Dallago, Bruno Stefano Lima ; da Silveira, Juliano Coelho ; Perecin, Felipe ; Pogue, Robert ; Carvalho, Juliana Lott

• Document type: Journal article
• Source: CYTOTHERAPY; v. 26, n. 10, p. 11-pg., 2024-09-24.

Abstract

Background and aims: Ovum pick-up (OPU) is an intrinsic step of in vitro fertilization procedures. Nevertheless, it can cause ovarian lesions and compromise female fertility in bovines. Recently, we have shown that intraovarian injection of adipose-derived mesenchymal stromal cells (AD-MSCs) effectively preserves ovarian function in bovines. Given that MSC-derived extracellular vesicles (MSC-EVs) have been shown to recapitulate several therapeutic effects attributed to AD-MSCs and that they present logistic and regulatory advantages compared to AD-MSCs, we tested whether MSC-EVs would also be useful to treat OPU-induced lesions. Methods: MSC-EVs were isolated from the secretome of bovine AD-MSCs, using ultrafiltration (UF) and ultracentrifugation methods. The MSC-EVs were characterized according to concentration and mean particle size, morphology, protein concentration and EV markers, miRNA, mRNA, long noncoding RNA profile, total RNA yield and potential for induction of the proliferation and migration of bovine ovarian stromal cells. We then investigated whether intraovarian injection of MSC-EVs obtained by UF would reduce the negative effects of acute OPU-induced ovarian lesions in bovines. To do so, 20 animals were divided into 4 experimental groups (n = 5), submitted to 4 OPU cycles and different experimental treatments including vehicle only (G1), MSC-EVs produced by 7.5 x 10(6) AD-MSCs (G2), MSC-EVs produced by 2.5 x 10(6) AD-MSCs (G3) or 3 doses of MSC-EVs produced by 2.5 x 10(6) AD-MSCs, injected after OPU sessions 1, 2 and 3 (G4). Results: Characterization of the MSC-EVs revealed that the size of the particles was similar in the different isolation methods; however, the UF method generated a greater MSC-EV yield. MSC-EVs processed by both methods demonstrated a similar ability to promote cell migration and proliferation in ovarian stromal cells. Considering the higher yield and lower complexity of the UF method, UF-MSC-EVs were used in the in vivo experiment. We evaluated three therapeutic regimens for cows subjected to OPU, noting that the group treated with three MSC-EV injections (G4) maintained oocyte production and increased in vitro embryo production, compared to G1, which presented compromised embryo production following the OPU-induced lesions. Conclusions: MSC-EVs have beneficial effects both on the migration and proliferation of ovarian stromal cells and on the fertility of bovines with follicular puncture injury in vivo. (c) 2024 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU)

• FAPESP's process: 22/01505-8 - Multi-user equipment approved in grant 21/06645-0: CytoFLEX System B3-R0-V3
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/06645-0 - Extracellular vesicles as a platform for diagnostic and manipulation of the in vitro embryo production system: the next generation in animal reproduction
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 19/23840-0 - The use of mesenchymal stem cells derived from extracellular vesicles to increase bovine female reproductive potencial: therapeutic effects comprehension and profitability analysis
• Grantee: Juliano Coelho da Silveira
• Support Opportunities: Regular Research Grants