| Full text | |
| Author(s): Show less - |
Rodrigues, Mariana Teixeira
;
Michelli, Ana Paula Picaro
;
Caso, Gustavo Felisola
;
de Oliveira, Paloma Ramos
;
Rodrigues-Junior, Dorival Mendes
;
Morale, Mirian Galliote
;
Machado Junior, Joel
;
Bortoluci, Karina Ramalho
;
Tamura, Rodrigo Esaki
;
da Silva, Tamiris Reissa Cipriano
;
Raminelli, Cristiano
;
Chau, Eric
;
Godin, Biana
;
Calil-Silveira, Jamile
;
Rubio, Ileana G. Sanchez
Total Authors: 15
|
| Document type: | Journal article |
| Source: | PHARMACEUTICALS; v. 16, n. 12, p. 18-pg., 2023-12-01. |
| Abstract | |
Anaplastic thyroid cancer (ATC) is an aggressive form of thyroid cancer (TC), accounting for 50% of total TC-related deaths. Although therapeutic approaches against TC have improved in recent years, the survival rate remains low, and severe adverse effects are commonly reported. However, unexplored alternatives based on natural compounds, such as lysicamine, an alkaloid found in plants with established cytotoxicity against breast and liver cancers, offer promise. Therefore, this study aimed to explore the antineoplastic effects of lysicamine in papillary TC (BCPAP) and ATC (HTH83 and KTC-2) cells. Lysicamine treatment reduced cell viability, motility, colony formation, and AKT activation while increasing the percentage of necrotic cells. The absence of caspase activity confirmed apoptosis-independent cell death. Necrostatin-1 (NEC-1)-mediated necrosome inhibition reduced lysicamine-induced necrosis in KTC-2, suggesting necroptosis induction via a reactive oxygen species (ROS)-independent mechanism. Additionally, in silico analysis predicted lysicamine target proteins, particularly those related to MAPK and TGF-beta signaling. Our study demonstrated lysicamine's potential as an antineoplastic compound in ATC cells with a proposed mechanism related to inhibiting AKT activation and inducing cell death. (AU) | |
| FAPESP's process: | 14/24549-4 - Congenital hypothyroidism due to thyroid disgenesis: whole exome investigation of molecular genetics and cromossomal alterations |
| Grantee: | Ileana Gabriela Sánchez de Rubió |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 23/02690-6 - Modulation of the JAK/STAT pathway in advanced thyroid cancer as a potential therapeutic target: effect on iodine uptake, tumor stem cell characteristics |
| Grantee: | Ileana Gabriela Sánchez de Rubió |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/15619-2 - Development of high capacity adenoviral system expressing antitumoral genes |
| Grantee: | Rodrigo Esaki Tamura |
| Support Opportunities: | Research Grants - Young Investigators Grants |