| Full text | |
| Author(s): |
Santos, Cerize S.
;
Lemos, Marcos P. O.
;
Betschart, Larissa M.
;
Baader, Wilhelm J.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Photochemistry and Photobiology; v. 100, n. 6, p. 16-pg., 2024-02-23. |
| Abstract | |
Antioxidant substances which can diminish the steady-state concentration of free radicals in vivo are important in the human dietary to diminish the deleterious effects of oxidative stress. As the potential of certain substances as antioxidants is difficult to be verified in vivo, simple chemical in vitro assays which test the potential of substances as antioxidants are of great importance for the screening of new antioxidants. These assays measure the capacity of a substance to suppress free radicals. We describe here an antiradical capacity assay, based on luminol chemiluminescence, in cationic micellar medium, allowing the capacity determination of hydrophobic compounds. The antiradical capacity of antioxidants is determined using the Trolox standard by the measurement of the light emission inhibition area caused by the addition of different antiradical concentrations. The obtained results are compared to the values determined using the scavenging of stable free radicals be the substances and shown to be similar for compounds like uric acid, rutin, and quercetin. However, for vitamin E, the luminol assay results in a considerably higher antiradical capacity than the assay with a stable free radical, which is rationalized by the higher reactivity of the radical generated in the luminol assay and a specific localization of vitamin E in the micellar medium. Hemin-catalyzed luminol chemiluminescence assay in micellar medium allows the determination of antiradical capacity of hydrophobic antioxidants giving rise to higher antiradical capacity valued for vitamin E than formerly determined.image (AU) | |
| FAPESP's process: | 14/22136-4 - Use of green solvents and their mixtures for optimizing chemical processes |
| Grantee: | Omar Abou El Seoud |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/13791-2 - Multi-user equipment approved in grant 2014/22136-4: FS5 Steady State Spectrometer, Edinburgh Instruments |
| Grantee: | Omar Abou El Seoud |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 23/06417-2 - Mechanisms of Organic Chemiluminescence |
| Grantee: | Joséf Wilhelm Baader |
| Support Opportunities: | Regular Research Grants |