Effect of experimental dentin etchants on dentin bond strength, metalloproteinase inhibition, and antibiofilm activity

Author(s)

Sahadi, Beatriz Ometto ; Sebold, Maicon ; Andre, Carolina Bosso ; Nima, Gabriel ; dos Santos, Andressa ; Damasceno, Marina ; Chiari, Souza de Carvalho ; Nascimento, Fabio Dupart ; Tersariol, Ivarne Luis dos Santos ; Giannini, Marcelo

• Document type: Journal article
• Source: Dental Materials; v. 40, n. 4, p. 12-pg., 2024-04-11.

Abstract

Objective: this study evaluated dentin microtensile bond strength ( mu TBS) and failure modes (at 24 h and one year), bonding interface regarding hybridization, surface morphology regarding demineralization, in situ metalloproteinase (MMP) activity, and antibacterial effect of three dentin etchants compared to 35% phosphoric acid (PA). Materials and Methods: The Adper Single Bond 2 adhesive (3 M Oral Care) was applied on moist dentin etched with PA (control) or on air-dried dentin etched with 3% aluminum nitrate + 2% oxalic acid (AN), 6.8% ferric oxalate + 10% citric acid (FO), or 10% citric acid (CA). The mu TBS test used 40 human teeth (n = 10). Failure modes and surface morphology were analyzed by scanning electron microscopy (n = 3), while bonding interface morphology and MMP activity were evaluated by laser scanning confocal microscopy (n = 3). Antibacterial activity was evaluated against S. Mutans biofilm by means of viable cells count (CFU/mL). Results: PA presented the highest bond strengths regardless of aging time. PA, AN, and CA showed stable bond strengths after one year of storage. Adhesive and mixed failures were predominant in all groups. Thin hybrid layers with short resin tags were observed for the experimental etchants. The AN -based etchant was able to inhibit MMP activity. All tested etchants presented antibacterial activity against S. Mutans biofilm. Significance: This study suggests different dentin etchants capable of inhibiting MMP activity while also acting as cavity disinfectants. (AU)

• FAPESP's process: 19/14973-7 - In vitro evaluation of the effect of experimental primers on dentin-adhesive bonding and dentin matrix matalloproteinase inhibition
• Grantee: Beatriz Ometto Sahadi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 21/11972-0 - Evaluation of the potential antimicrobial effect of experimental primers containing flavonoids on cavity disinfection and mechanical properties of dentin affected by caries using a microcosm biofilm model
• Grantee: Beatriz Ometto Sahadi
• Support Opportunities: Scholarships in Brazil - Doctorate