| Full text | |
| Author(s): Show less - |
da Silva-Januario, Mara E.
;
da Costa, Cristina S.
;
Tavares, Lucas A.
;
Oliveira, Ana K.
;
Januario, Yunan C.
;
de Carvalho, Andreia N.
;
Cassiano, Murilo H. A.
;
Rodrigues, Roger L.
;
Miller, Michael E.
;
Palameta, Soledad
;
Arns, Clarice W.
;
Arruda, Eurico
;
Leme, Adriana F. Paes
;
daSilva, Luis L. P.
Total Authors: 14
|
| Document type: | Journal article |
| Source: | MOLECULAR & CELLULAR PROTEOMICS; v. 22, n. 12, p. 17-pg., 2023-12-04. |
| Abstract | |
Extracellular vesicles (EVs) are biomolecule carriers for intercellular communication in health and disease. Nef is a HIV virulence factor that is released from cells within EVs and is present in plasma EVs of HIV -1 infected individuals. We performed a quantitative proteomic analysis to fully characterize the Nef-induced changes in protein composition of T cell -derived EVs and identify novel host targets of HIV. Several proteins with well -described roles in infection or not previously associated with HIV pathogenesis were specifically modulated by Nef in EVs. Among the downregulated proteins are the interferon -induced transmembrane 1, 2, and 3 (IFITM1-3) proteins, broadspectrum antiviral factors known to be cell -to -cell transferable by EVs. We demonstrate that Nef depletes IFITM13 from EVs by excluding these proteins from the plasma membrane and lipid rafts, which are sites of EVs biogenesis in T cells. Our data establish Nef as a modulator of EVs' global protein content and as an HIV factor that antagonizes IFITMs. (AU) | |
| FAPESP's process: | 22/15928-8 - Study of the molecular mechanisms used by the HIV-1 Nef protein to neutralize host cell defense strategies |
| Grantee: | Luis Lamberti Pinto da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/00297-7 - Study of the effects of HIV-1 Nef on the host-cell endomembrane system and their implications in viral pathogenesis |
| Grantee: | Luis Lamberti Pinto da Silva |
| Support Opportunities: | Regular Research Grants |