Effectiveness and safety of biosilicate-enhanced bleaching gels on enamel with early erosion lesion

Author(s)

Dascanio, Rafael ; Ribeiro, Rafael Antonio de Oliveira ; Coelho, Camila Siqueira Silva ; Souza, Marina Trevelin ; Kury, Matheus ; Zanotto, Edgar Dutra ; Costa, Carlos Alberto de Souza ; Cavalli, Vanessa

• Document type: Journal article
• Source: Journal of Esthetic and Restorative Dentistry; v. 36, n. 10, p. 14-pg., 2024-06-09.

Abstract

Aim: This study evaluated the efficacy and cytotoxicity of 35% hydrogen peroxide (HP) gel incorporated with 10% (w/w) biosilicate (BioS) on sound enamel and early-stage enamel erosion lesions. Methods: Discs of enamel/dentin were selected, subjected to erosive cycles (0.3% citric acid, pH 2.6), and treated with (n = 8): HP (35% HP, positive control); HP_BioS [carboxymethyl cellulose (CMC) + HP + BioS]; BioS (CMC + BioS); CMC (negative control). The discs were adapted to artificial pulp chambers with the enamel exposed for bleaching, and the dentin facing toward the culture medium (Dulbecco's modified Eagle's medium [DMEM]). Bleaching was performed in three 30-min sessions at 7-day intervals. After bleaching, the diffusion product (DMEM extract + diffused HP) was pipetted onto MDPC-23 odontoblastic cell line and inoculated. Color parameters (Delta L, Delta a, Delta b), color change (Delta E-00), and changes in whiteness index (Delta WID) were determined before (T-0) and after the last bleaching session (T-3). Cell viability (MTT, %), H(2)O(2 )diffusion (mu g/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, in confocal microscopy) were assessed (ANOVA/Tukey; alpha = 0.05). Results: No difference in Delta L, Delta a, Delta b, Delta E-00, and Delta WI(D )were found between HP and HP_BioS (p > 0.05). The incorporation of BioS decreased the HP diffusion into the substrates and mitigated oxidative stress in early-stage eroded enamel (p < 0.05). HP_BioS presented significantly higher cell viability compared with HP under erosion conditions. Live/dead assay indicated that BioS_HP maintained viability with larger clusters of viable cells. Conclusion: Incorporating BioS into HP maintained bleaching effectiveness, favored cell viability, reduced the oxidative stress, and the cytotoxicity in teeth with early-stage erosion. Clinical Significance: BioS formulation showed promising results for reducing cytotoxicity in patients seeking tooth bleaching and presenting undetectable early-stage erosion. (AU)

• FAPESP's process: 23/03511-8 - In vitro and in situ evaluation of the efficacy of bleaching gels containing hydrogen peroxide associated with Biosilicate® doped with Manganese Oxide and activated with violet light
• Grantee: Rafael Dascanio
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 20/06782-4 - Development of experimental bleaching agents with bioactive characteristics: analysis of the physical properties of bleached enamel and cell biocompatibility
• Grantee: Vanessa Cavalli Gobbo
• Support Opportunities: Regular Research Grants