| Full text | |
| Author(s): |
Ribeiro, Luiz Fernando
;
Babadopulos, Tainah
;
de Oliveira, Mariana G.
;
Nishimaru, Flavio
;
Zatz, Roberto
;
Elias, Rosilene Motta
;
Moraes, Odorico
;
Moraes, Elisabete
;
Peterson, Larryn W.
;
De Nucci, Gilberto
Total Authors: 10
|
| Document type: | Journal article |
| Source: | BIOMEDICAL CHROMATOGRAPHY; v. 38, n. 8, p. 17-pg., 2024-05-26. |
| Abstract | |
The aim of this study was to develop a high-performance liquid chromatography-tandem mass spectrometry method for the determination of 6-cyanodopamine, 6-nitrodopamine, 6-nitrodopa, 6-nitroadrenaline and 6-bromodopamine in human plasma samples. Strata-X 33 mu m solid-phase extraction cartridges were used for the extraction of the catecholamines from human plasma samples. The catecholamines were separated in a 150 x 3 mm Shim-pack GIST C18-AQ column with 3 mu m particle size, placed in an oven at 40 degrees C and perfused with 82% mobile phase A (acetonitrile-H2O; 90:10, v/v) + 0.4% acetic acid and 18% mobile phase B (deionized H2O) + 0.2% formic acid at a flow rate of 340 mu l/min in isocratic mode. The injected volume was 4 mu l and the run lasted 4 min. The method was linear from 0.1 to 20 ng/ml and the lower limit of quantification was 0.1 ng/ml for all analytes. The method was applied to evaluate the plasma levels of catecholamines in plasma of patients with chronic kidney disease and allowed the detection for the first time of circulating levels of the novel catecholamines 6-bromodopamine and 6-cyanodopamine. (AU) | |
| FAPESP's process: | 23/01267-2 - An evolutionary perspective on endothelial cardiovascular control in vertebrates and its functions |
| Grantee: | Gilberto de Nucci |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 23/09792-9 - Evaluation of 6-Nitrodopamine Effects on Renal and Urogenital Function |
| Grantee: | Mariana Gonçalves de Oliveira Taranto |
| Support Opportunities: | Scholarships in Brazil - Support Program for Fixating Young Doctors |
| FAPESP's process: | 23/15165-7 - Development of Liquid Chromatography methods coupled to Tandem Mass Spectrometry (LC-MS/MS) for monitoring the metabolites of new catecholamines |
| Grantee: | Luiz Fernando Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Support Program for Fixating Young Doctors |