Peri-implantitis increases the risk of medication-related osteonecrosis of the jaws associated with osseointegrated implants in rats treated with zoledronate

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Souza, Eduardo Quintao Manhanini ; Toro, Luan Felipe ; Ganzaroli, Vinicius Franzao ; Freire, Jessica de Oliveira Alvarenga ; Matsumoto, Mariza Akemi ; Casatti, Claudio Aparecido ; Cintra, Luciano Tavares angelo ; Buchaim, Rogerio Leone ; Issa, Joao Paulo Mardegan ; Garcia, Valdir Gouveia ; Theodoro, Leticia Helena ; Ervolino, Edilson

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 14, n. 1, p. 15-pg., 2024-01-05.

Abstract

This study evaluated the peri-implant tissues under normal conditions and under the influence of experimental peri-implantitis (EPI) in osseointegrated implants installed in the maxillae of rats treated with oncologic dosage of zoledronate. Twenty-eight senescent female rats underwent the extraction of the upper incisor and placement of a titanium dental implant (DI). After eight weeks was installated a transmucosal healing screw on DI. After nine weeks, the following groups were formed: VEH, ZOL, VEH-EPI and ZOL-EPI. From the 9th until the 19th, VEH and VEH-EPI groups received vehicle and ZOL and ZOL-EPI groups received zoledronate. At the 14th week, a cotton ligature was installed around the DI in VEH-EPI and ZOL-EPI groups to induce the EPI. At the 19th week, euthanasia was performed, and the maxillae were processed so that at the implanted sites were analyzed: histological aspects and the percentage of total bone tissue (PTBT) and non-vital bone tissue (PNVBT), along with TNF alpha, IL-1 beta, VEGF, OCN and TRAP immunolabeling. ZOL group presented mild persistent peri-implant inflammation, higher PNVBT and TNF alpha and IL-1 beta immunolabeling, but lower for VEGF, OCN and TRAP in comparison with VEH group. ZOL-EPI group exhibited exuberant peri-implant inflammation, higher PNVBT and TNF alpha and IL-1 beta immunolabeling when compared with ZOL and VEH-EPI groups. Zoledronate disrupted peri-implant environment, causing mild persistent inflammation and increasing the quantity of non-vital bone tissue. Besides, associated with the EPI there were an exacerbated inflammation and even greater increase in the quantity of non-vital bone around the DI, which makes this condition a risk factor for medication-related osteonecrosis of the jaws. (AU)

• FAPESP's process: 17/16364-2 - Evaluation of the peri-implant repair process in rats treated with oncologic dose or osteoporotic dose of zoledronate
• Grantee: Luan Felipe Toro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/17769-1 - Treatment with antiresorptive drugs: action on osseointegrated implants, effects on experimental peri-implantitis and interference with the response of peri-implantary tissues through different local therapies
• Grantee: Edilson Ervolino
• Support Opportunities: Regular Research Grants