| Full text | |
| Author(s): Show less - |
Villena-Rueda, Beatriz Enguidanos
;
Kajitani, Gustavo Satoru
;
Ota, Vanessa Kiyomi
;
Honorato-Mauer, Jessica
;
Santoro, Marcos Leite
;
Bugiga, Amanda Victoria Gomes
;
Rosa, Joice Santos
;
Asprino, Paula Fontes
;
Meneghetti, Paula
;
Torrecilhas, Ana Claudia
;
Intasqui, Paula
;
Bertolla, Ricardo Pimenta
;
Foresti, Maira Licia
;
Naffah-Mazzacoratti, Maria da Graca
;
Mello, Luiz Eugenio Araujo de Moraes
;
Belangero, Sintia Iole
Total Authors: 16
|
| Document type: | Journal article |
| Source: | Molecular Neurobiology; v. 61, n. 11, p. 13-pg., 2024-04-26. |
| Abstract | |
Traumatic brain injury (TBI) is a prevalent and debilitating condition, which often leads to the development of post-traumatic epilepsy (PTE), a condition that yet lacks preventive strategies. Biperiden, an anticholinergic drug, is a promising candidate that has shown efficacy in murine models of PTE. MicroRNAs (miRNAs), small regulatory RNAs, can help in understanding the biological basis of PTE and act as TBI- and PTE-relevant biomarkers that can be detected peripherally, as they are present in extracellular vesicles (EVs) that cross the blood-brain barrier. This study aimed to investigate miRNAs in serum EVs from patients with TBI, and their association with biperiden treatment and PTE. Blood samples of 37 TBI patients were collected 10 days after trauma and treatment initiation in a double-blind clinical trial. A total of 18 patients received biperiden, with three subjects developing PTE, and 19 received placebo, with two developing PTE. Serum EVs were characterized by size distribution and protein profiling, followed by high-throughput sequencing of the EV miRNome. Differential expression analysis revealed no significant differences in miRNA expression between TBI patients with and without PTE. Interestingly, miR-9-5p displayed decreased expression in biperiden-treated patients compared to the placebo group. This miRNA regulates genes enriched in stress response pathways, including axonogenesis and neuronal death, relevant to both PTE and TBI. These findings indicate that biperiden may alter miR-9-5p expression in serum EVs, which may play a role in TBI resolution. (AU) | |
| FAPESP's process: | 23/04184-0 - Search for miRNAs from extracellular vesicles relevant to epileptogenesis and polygenic risk score of patients who suffered traumatic brain injury |
| Grantee: | Beatriz Enguidanos Villena Rueda |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 22/14055-0 - Evaluation of the prognostic potential of MicroRNAs in the development of epilepsy after traumatic brain injury |
| Grantee: | Gustavo Satoru Kajitani |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/24561-5 - Epileptogenesis, biomarkers and post-traumatic epilepsy |
| Grantee: | Luiz Eugenio Araujo de Moraes Mello |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/14564-0 - Search for miRNAs from exosomes relevant to epileptogenesis |
| Grantee: | Beatriz Enguidanos Villena Rueda |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |